• Western blot analysis of lysates from COS7 cells, using IkappaB-alpha (Phospho-Ser32/Ser36) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunofluorescence analysis of LOVO cells, using IkappaB-alpha (Phospho-Ser32/Ser36) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IkappaB-alpha (Phospho-Ser32/Ser36) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-I Kappa B-Alpha (S32/S36) Polyclonal Antibody
  • Western blot analysis of COS7 cells using Phospho-I Kappa B-Alpha (S32/S36) Polyclonal Antibody
  • Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, I Kappa B-Alpha (phospho Ser32/S36) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, I Kappa B-Alpha (phospho Ser32/S36) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, I Kappa B-Alpha (phospho Ser32/S36) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1, I Kappa B-Alpha (phospho Ser32/S36) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, I Kappa B-Alpha (phospho Ser32/S36) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1, I Kappa B-Alpha (phospho Ser32/S36) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, I Kappa B-Alpha (phospho Ser32/S36) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, I Kappa B-Alpha (phospho Ser32/S36) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunofluorescence analysis of rat-spleen tissue. 1, I Kappa B-Alpha (phospho Ser32/S36) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-NFKBIA-Ser32/S36 antibody (15-64 aa) (STJ90311)

SKU:
STJ90311

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-NF-kappa-B inhibitor alpha-Ser32/S36 (15-64 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NFKBIA
Gene ID: 4792
Uniprot ID: IKBA_HUMAN
Immunogen Region: 15-64 aa
Specificity: Phospho-I Kappa B-Alpha (S32/S36) Polyclonal Antibody detects endogenous levels of I Kappa B-Alpha protein only when phosphorylated at S32/S36.
Immunogen: The antiserum was produced against synthesized peptide derived from the human IkappaB-alpha around the phosphorylation site of Ser32/Ser36 at the amino acid range 15-64
Function Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals. On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Protein Name Nf-Kappa-B Inhibitor Alpha
I-Kappa-B-Alpha
Ikb-Alpha
Ikappabalpha
Major Histocompatibility Complex Enhancer-Binding Protein Mad3
Database Links Reactome: R-HSA-1169091
Reactome: R-HSA-1810476
Reactome: R-HSA-202424
Reactome: R-HSA-209560
Reactome: R-HSA-2871837
Reactome: R-HSA-445989
Reactome: R-HSA-4755510
Reactome: R-HSA-5603029
Reactome: R-HSA-5607764
Reactome: R-HSA-5689880
Reactome: R-HSA-9020702
Reactome: R-HSA-933542
Reactome: R-HSA-9692916
Cellular Localisation Cytoplasm
Nucleus
Shuttles Between The Nucleus And The Cytoplasm By A Nuclear Localization Signal (Nls) And A Crm1-Dependent Nuclear Export
Alternative Antibody Names Anti-Nf-Kappa-B Inhibitor Alpha antibody
Anti-I-Kappa-B-Alpha antibody
Anti-Ikb-Alpha antibody
Anti-Ikappabalpha antibody
Anti-Major Histocompatibility Complex Enhancer-Binding Protein Mad3 antibody
Anti-NFKBIA antibody
Anti-IKBA antibody
Anti-MAD3 antibody
Anti-NFKBI antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance