• Western blot analysis of extracts of HeLa cells, using Phospho-JNK1/2/3-T183/T183/T221 antibody (STJ113512) at 1:3000 dilution. HeLa cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of 293T, using Phospho-JNK1/2/3-T183/T183/T221 antibody (A23099) at 1:1000 dilution. 293T cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of 293T, using Phospho-JNK1/2/3-T183/T183/T221 antibody (A23099) at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of C6, using Phospho-JNK1/2/3-T183/T183/T221 antibody (A23099) at 1:1000 dilution. C6 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of C6, using Phospho-JNK1/2/3-T183/T183/T221 antibody (A23099) at 1:1000 dilution. C6 cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 20 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Phospho-JNK1/2/3-T183/T183/T221 rabbit monoclonal antibody (STJ113512) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using Phospho-JNK1/2/3-T183/T183/T221 rabbit monoclonal antibody (STJ113512) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung squamous carcinoma tissue using Phospho-JNK1/2/3-T183/T183/T221 rabbit monoclonal antibody (STJ113512) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using Phospho-JNK1/2/3-T183/T183/T221 rabbit monoclonal antibody (STJ113512) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse colon using Phospho-JNK1/2/3-T183/T183/T221 rabbit monoclonal antibody (STJ113512) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using Phospho-JNK1/2/3-T183/T183/T221 rabbit monoclonal antibody (STJ113512) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat liver using Phospho-JNK1/2/3-T183/T183/T221 rabbit monoclonal antibody (STJ113512) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH-3T3 cells using Phospho-JNK1/2/3-T183/T183/T221 antibody (STJ113512). NIH-3T3 cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes after serum-starvation overnight. Blue: DAPI for nuclear staining.

Anti-Phospho-MAPK8/MAPK9/MAPK10-T183/T183/T221 antibody [S2MR] (STJ113512)

SKU:
STJ113512

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Phospho-JNK1/2/3-T183/T183/T221 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAPK10
MAPK9
5602
5601
5599
MK10_HUMAN
MK09_HUMAN
MK08_HUMAN
Immunogen: A synthetic phosphorylated peptide around T183 of human JNK1 (P45983).
Immunogen Sequence: MMTPY
Tissue Specificity
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase 10 antibody
Anti-Map Kinase 10 antibody
Anti-Mapk 10 antibody
Anti-Map Kinase P49 3f12 antibody
Anti-Stress-Activated Protein Kinase 1b antibody
Anti-Sapk1b antibody
Anti-Stress-Activated Protein Kinase Jnk3 antibody
Anti-C-Jun N-Terminal Kinase 3 antibody
Anti-MAPK10 antibody
Anti-JNK3 antibody
Anti-JNK3A antibody
Anti-PRKM10 antibody
Anti-SAPK1B antibody
Anti-Mitogen-Activated Protein Kinase 9 antibody
Anti-Map Kinase 9 antibody
Anti-Mapk 9 antibody
Anti-Jnk-55 antibody
Anti-Stress-Activated Protein Kinase 1a antibody
Anti-Sapk1a antibody
Anti-Stress-Activated Protein Kinase Jnk2 antibody
Anti-C-Jun N-Terminal Kinase 2 antibody
Anti-MAPK9 antibody
Anti-JNK2 antibody
Anti-PRKM9 antibody
Anti-SAPK1A antibody
Anti-Mitogen-Activated Protein Kinase 8 antibody
Anti-Map Kinase 8 antibody
Anti-Mapk 8 antibody
Anti-Jnk-46 antibody
Anti-Stress-Activated Protein Kinase 1c antibody
Anti-Sapk1c antibody
Anti-Stress-Activated Protein Kinase Jnk1 antibody
Anti-C-Jun N-Terminal Kinase 1 antibody
Anti-MAPK8 antibody
Anti-JNK1 antibody
Anti-PRKM8 antibody
Anti-SAPK1 antibody
Anti-SAPK1C antibody
Anti-Mitogen-Activated Protein Kinase 10 antibody
Anti-Map Kinase 10 antibody
Anti-Mapk 10 antibody
Anti-Map Kinase P49 3f12 antibody
Anti-Stress-Activated Protein Kinase 1b antibody
Anti-Sapk1b antibody
Anti-Stress-Activated Protein Kinase Jnk3 antibody
Anti-C-Jun N-Terminal Kinase 3 antibody
Anti-MAPK10 antibody
Anti-JNK3 antibody
Anti-JNK3A antibody
Anti-PRKM10 antibody
Anti-SAPK1B antibody
Anti-Mitogen-Activated Protein Kinase 9 antibody
Anti-Map Kinase 9 antibody
Anti-Mapk 9 antibody
Anti-Jnk-55 antibody
Anti-Stress-Activated Protein Kinase 1a antibody
Anti-Sapk1a antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance