• Western blot analysis of extracts of various cell lines, using Phospho-Histone H3-S10 rabbit monoclonal antibody (STJ11102549) at 1:1000 dilution. HeLa, NIH/3T3 and C6 cells were treated by nocodazole (50 ng/mL) at 37 °C for 20 hours or treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Anti-Phospho-Histone H3-S10 antibody [S9MR] (STJ11102549)
  • Immunohistochemistry of paraffin-embedded human oophoroma using Phospho-Histone H3-S10 antibody (STJ11102549) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 50 mM Tris/EDTA buffer pH 8. 0 before commencing with immunohistochemistry staining protocol.
  • Confocal imaging of C6 cells using Phospho-Histone H3-S10 Rabbit monoclonal antibody (STJ11102549, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-Histone H3-S10 antibody (STJ11102549) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 50 mM Tris/EDTA buffer pH 8. 0 before commencing with immunohistochemistry staining protocol.
  • Confocal imaging of HeLa cells using Phospho-Histone H3-S10 Rabbit monoclonal antibody (STJ11102549, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunofluorescence analysis of C6 cells using Phospho-Histone H3-S10 antibody (STJ11102549). Blue: DAPI for nuclear staining.
  • Confocal imaging of NIH/3T3 cells using Phospho-Histone H3-S10 Rabbit monoclonal antibody (STJ11102549, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunofluorescence analysis of NIH-3T3 cells using Phospho-Histone H3-S10 antibody (STJ11102549). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Phospho-Histone H3-S10 in paraffin-embedded mouse kidney using Phospho-Histone H3-S10 Rabbit monoclonal antibody (STJ11102549) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 50 mM Tris/EDTA buffer pH 8. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Phospho-Histone H3-S10 in paraffin-embedded human oophoroma using Phospho-Histone H3-S10 Rabbit monoclonal antibody (STJ11102549) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 50 mM Tris/EDTA buffer pH 8. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from HeLa, NIH/3T3, C6 cells using Phospho-Histone H3-S10 Rabbit monoclonal antibody (STJ11102549) at 1:1000 dilution. HeLa, NIH/3T3 and C6 cells were treated by nocodazole (50 ng/mL) at 37 °C for 20 hours or treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 1s.

Anti-Phospho-Histone H3-S10 antibody [S9MR] (STJ11102549)

SKU:
STJ11102549

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Phospho-Histone H3-S10 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic phosphorylated peptide around S10 of human Histone H3 (P68431).
Immunogen Sequence: ARKST
Background Enables RNA binding activity and cadherin binding activity. Predicted to be involved in positive regulation of Ras protein signal transduction and signal transduction. Located in endoplasmic reticulum and mitochondrial nucleoid.

Information sourced from Uniprot.org

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