• Western blot analysis of extracts of Jurkat cells, using Phospho-ERK1-T202 + ERK2-T185 rabbit monoclonal antibody (STJ11102556) at 1:1000 dilution. Jurkat cells were treated by PMA/TPA (200 nM) at 37 °C for 10 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using Phospho-ERK1-T202 + ERK2-T185 Rabbit monoclonal antibody (STJ11102556) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of NIH/3T3 cells, using Phospho-ERK1-T202 + ERK2-T185 rabbit monoclonal antibody (STJ11102556) at 1:1000 dilution. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1S.
  • Immunohistochemistry analysis of paraffin-embedded human brain using Phospho-ERK1-T202 + ERK2-T185 Rabbit monoclonal antibody (STJ11102556) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat lung using Phospho-ERK1-T202 + ERK2-T185 antibody (STJ11102556) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of NIH/3T3 cells, using Phospho-ERK1-T202 + ERK2-T185 Rabbit monoclonal antibody (STJ11102556) at 1:1000 dilution. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1S.
  • Immunohistochemistry of paraffin-embedded human appendix using Phospho-ERK1-T202 + ERK2-T185 antibody (STJ11102556) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of Jurkat cells, using Phospho-ERK1-T202 + ERK2-T185 Rabbit monoclonal antibody (STJ11102556) at 1:1000 dilution. Jurkat cells were treated by PMA/TPA (200 nM) at 37 °C for 10 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry of paraffin-embedded mouse liver using Phospho-ERK1-T202 + ERK2-T185 antibody (STJ11102556) at dilution of 1:100 (40x lens).

Anti-Phospho-ERK1/ERK2-T202/T185 antibody [S6MR] (STJ11102556)

SKU:
STJ11102556

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Phospho-ERK1/ERK2-T202/T185 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic phosphorylated peptide around T202 of human ERK1 (P27361).
Immunogen Sequence: FLTEY
Background This gene encodes one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported.

Information sourced from Uniprot.org

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