• Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Phospho-ERK1/2 Y222/205 Mouse mAb diluted at 1:200.
  • Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using Phospho-ERK1/2 Y222/205 Mouse mAb diluted at 1:200

Anti-Phospho-ERK 1/2 (Y222/205) antibody [4G3] (STJ97738)

SKU:
STJ97738

Current Stock:
Host: Mouse
Applications: IHC-P
Reactivity: Human, Rat, Mouse
Short Description: Mouse monoclonal anti-Phospho-ERK 1/2 (Y222/205) antibody is suitable for use in Immunohistochemistry.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Monoclonal
Clone ID: 4G3
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using a specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG1
Dilution Range: IHC-p 1:100-200
Specificity: Phospho-ERK 1/2 (Y222/205) protein detects endogenous levels of MAPK1/MAPK3
Uniprot ID: MK03_HUMAN
Gene ID: 5595
Gene Symbol: MAPK3
Immunogen: synthetic peptide derived from Phospho-ERK 1/2 (Y222/205)
Immunogen Region: 140-220 aa
Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC).as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.
Protein Name Mitogen-Activated Protein Kinase 3
Map Kinase 3
Mapk 3
Ert2
Extracellular Signal-Regulated Kinase 1
Erk-1
Insulin-Stimulated Map2 Kinase
Map Kinase Isoform P44
P44-Mapk
Microtubule-Associated Protein 2 Kinase
P44-Erk1
Database Links Reactome: R-HSA-110056
Reactome: R-HSA-112409
Reactome: R-HSA-1169408
Reactome: R-HSA-1295596
Reactome: R-HSA-162658 P27361-3
Reactome: R-HSA-170968
Reactome: R-HSA-198753
Reactome: R-HSA-202670
Reactome: R-HSA-2029482
Reactome: R-HSA-2559580
Reactome: R-HSA-2559582
Reactome: R-HSA-2559585
Reactome: R-HSA-2871796
Reactome: R-HSA-3371453
Reactome: R-HSA-375165
Reactome: R-HSA-444257
Reactome: R-HSA-445144
Reactome: R-HSA-450341
Reactome: R-HSA-456926
Reactome: R-HSA-5654726
Reactome: R-HSA-5654727
Reactome: R-HSA-5654732
Reactome: R-HSA-5654733
Reactome: R-HSA-5663213
Reactome: R-HSA-5668599
Reactome: R-HSA-5673001
Reactome: R-HSA-5674135
Reactome: R-HSA-5674499
Reactome: R-HSA-5675221
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-6811558
Reactome: R-HSA-73728
Reactome: R-HSA-74749
Reactome: R-HSA-879415
Reactome: R-HSA-881907
Reactome: R-HSA-8939211
Reactome: R-HSA-8940973
Reactome: R-HSA-8943724
Reactome: R-HSA-9627069
Reactome: R-HSA-9634638
Reactome: R-HSA-9635465
Reactome: R-HSA-9649948
Reactome: R-HSA-9652169
Reactome: R-HSA-9656223
Reactome: R-HSA-9664422
Reactome: R-HSA-982772
Cellular Localisation Cytoplasm
Nucleus
Membrane
Caveola
Autophosphorylation At Thr-207 Promotes Nuclear Localization
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase 3 antibody
Anti-Map Kinase 3 antibody
Anti-Mapk 3 antibody
Anti-Ert2 antibody
Anti-Extracellular Signal-Regulated Kinase 1 antibody
Anti-Erk-1 antibody
Anti-Insulin-Stimulated Map2 Kinase antibody
Anti-Map Kinase Isoform P44 antibody
Anti-P44-Mapk antibody
Anti-Microtubule-Associated Protein 2 Kinase antibody
Anti-P44-Erk1 antibody
Anti-MAPK3 antibody
Anti-ERK1 antibody
Anti-PRKM3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance