• Western blot analysis of extracts of various cell lines, using Phospho-eEF2-T56 antibody (STJ11100973) at 1:1000 dilution. NIH/3T3 cells were treated by Forskolin (30uM) for 30 minutes after serum-starvation overnight. C6 cells were treated by Forskolin (10uM) for 1 hour after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of lysates from 293T cells, using Phospho-eEF2-T56 Rabbit polyclonal antibody (STJ11100973) at 1:3000 dilution. 293T cells were treated by 20% FBS at 37 °C for 20 minutes after serum-starvation overnightSecondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-Phospho-EEF2-T56 antibody (STJ11100973)

SKU:
STJ11100973

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-EEF2-T56 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EEF2
Gene ID: 1938
Uniprot ID: EF2_HUMAN
Immunogen: A synthetic phosphorylated peptide around T56 of human Phospho-eEF2-T56 (NP_001952.1).
Immunogen Sequence: TRFTD
Post Translational Modifications Phosphorylation by EF-2 kinase completely inactivates EF-2.it requires prior phosphorylation by CDK2 at Ser-595 during mitotic prometaphase. Phosphorylation by CSK promotes SUMOylation, proteolytic cleavage, and nuclear translocation if the C-terminal fragment. Diphthamide is 2-3-carboxyamido-3-(trimethyl-ammonio)propylhistidine. (Microbial infection) Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis. ISGylated. Proteolytically processed at two sites following phosphorylation by CSK. SUMOylated following phosphorylation by CSK, promotes proteolytic cleavage.
Function Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Protein Name Elongation Factor 2
Ef-2
Database Links Reactome: R-HSA-156902
Reactome: R-HSA-5336415
Reactome: R-HSA-5358493
Reactome: R-HSA-6798695
Reactome: R-HSA-8876725
Cellular Localisation Cytoplasm
Nucleus
Phosphorylation By Csk Promotes Cleavage And Sumoylation-Dependent Nuclear Translocation Of The C-Terminal Cleavage Product
Alternative Antibody Names Anti-Elongation Factor 2 antibody
Anti-Ef-2 antibody
Anti-EEF2 antibody
Anti-EF2 antibody

Information sourced from Uniprot.org

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