Anti-Phospho-CDK2-Y15 antibody [ARC1550] (STJ11102594)

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STJ11102594

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Host: Rabbit
Applications: WB
Reactivity: Human, Mouse
Short Description: Rabbit monoclonal anti-Phospho-CDK2-Y15 antibody is suitable for use in Western Blot.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Monoclonal
Clone ID: ARC1550
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
Uniprot ID: CDK2_HUMAN
Gene ID: 1017
Gene Symbol: CDK2
Immunogen: A phospho specific peptide corresponding to residues surrounding Y15 of human CDK2
Post Translational Modifications Phosphorylated at Thr-160 by CDK7 in a CAK complex. Phosphorylation at Thr-160 promotes kinase activity, whereas phosphorylation at Tyr-15 by WEE1 reduces slightly kinase activity. Phosphorylated on Thr-14 and Tyr-15 during S and G2 phases before being dephosphorylated by CDC25A. Nitrosylated after treatment with nitric oxide (DETA-NO).
Function Serine/threonine-protein kinase involved in the control of the cell cycle.essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression.controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2.activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1. Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis.regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. CTNNB1 phosphorylation regulates insulin internalization. Phosphorylates FOXP3 and negatively regulates its transcriptional activity and protein stability. Phosphorylates CDK2AP2. Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks.
Protein Name Cyclin-Dependent Kinase 2
Cell Division Protein Kinase 2
P33 Protein Kinase
Database Links Reactome: R-HSA-1538133
Reactome: R-HSA-171319
Reactome: R-HSA-176187
Reactome: R-HSA-176408
Reactome: R-HSA-187577
Reactome: R-HSA-2559582
Reactome: R-HSA-2559586
Reactome: R-HSA-5693607
Reactome: R-HSA-6804116
Reactome: R-HSA-6804756
Reactome: R-HSA-6804757
Reactome: R-HSA-68911
Reactome: R-HSA-68949
Reactome: R-HSA-68962
Reactome: R-HSA-69017
Reactome: R-HSA-69200
Reactome: R-HSA-69202
Reactome: R-HSA-69231
Reactome: R-HSA-69273
Reactome: R-HSA-69563
Reactome: R-HSA-69656
Reactome: R-HSA-8849470
Reactome: R-HSA-912446
Reactome: R-HSA-9616222
Reactome: R-HSA-9661069
Reactome: R-HSA-983231
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Nucleus
Cajal Body
Endosome
Localized At The Centrosomes In Late G2 Phase After Separation Of The Centrosomes But Before The Start Of Prophase
Nuclear-Cytoplasmic Trafficking Is Mediated During The Inhibition By 1
25-(Oh)(2)D(3)
Alternative Antibody Names Anti-Cyclin-Dependent Kinase 2 antibody
Anti-Cell Division Protein Kinase 2 antibody
Anti-P33 Protein Kinase antibody
Anti-CDK2 antibody
Anti-CDKN2 antibody

Information sourced from Uniprot.org

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