• Western blot analysis of extracts of various cell lines, using Phospho-CDC37-S13 rabbit monoclonal antibody (STJ11102637) at 1:1000 dilution. Both 293T cells and NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of C6 cells, using Phospho-CDC37-S13 Rabbit monoclonal antibody (STJ11102637) at 1:1000 dilution. C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of extracts of C6 cells, using Phospho-CDC37-S13 rabbit monoclonal antibody (STJ11102637) at 1:1000 dilution. C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of extracts of various cell lines, using Phospho-CDC37-S13 Rabbit monoclonal antibody (STJ11102637) at 1:1000 dilution. Both 293T cells and NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-CDC37-S13 antibody [S7MR] (STJ11102637)

SKU:
STJ11102637

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Phospho-CDC37-S13 is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDC37
Gene ID: 11140
Uniprot ID: CDC37_HUMAN
Immunogen: A synthetic phosphorylated peptide around S13 of human CDC37 (Q16543).
Immunogen Sequence: EVSDD
Post Translational Modifications Constitutively sumoylated by UBE2I.
Function Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity. Inhibits HSP90AA1 ATPase activity.
Protein Name Hsp90 Co-Chaperone Cdc37
Hsp90 Chaperone Protein Kinase-Targeting Subunit
P50cdc37 Cleaved Into - Hsp90 Co-Chaperone Cdc37 - N-Terminally Processed
Database Links Reactome: R-HSA-1227986
Reactome: R-HSA-1236382
Reactome: R-HSA-5637810
Reactome: R-HSA-5675482
Reactome: R-HSA-8863795
Reactome: R-HSA-9013418
Reactome: R-HSA-9634285
Reactome: R-HSA-9652282
Reactome: R-HSA-9664565
Reactome: R-HSA-9665233
Reactome: R-HSA-9665244
Reactome: R-HSA-9665245
Reactome: R-HSA-9665246
Reactome: R-HSA-9665247
Reactome: R-HSA-9665249
Reactome: R-HSA-9665250
Reactome: R-HSA-9665251
Reactome: R-HSA-9665348
Reactome: R-HSA-9665686
Reactome: R-HSA-9665737
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Hsp90 Co-Chaperone Cdc37 antibody
Anti-Hsp90 Chaperone Protein Kinase-Targeting Subunit antibody
Anti-P50cdc37 Cleaved Into - Hsp90 Co-Chaperone Cdc37 - N-Terminally Processed antibody
Anti-CDC37 antibody
Anti-CDC37A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance