Anti-Phospho-BTK-Y223 antibody [ARC0307] (STJ11102633)


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Host: Rabbit
Applications: WB
Reactivity: Human, Mouse, Rat
Short Description: Rabbit monoclonal anti-Phospho-BTK-Y223 antibody is suitable for use in Western Blot.
Clonality: Monoclonal
Clone ID: ARC0307
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
Gene ID: 695
Gene Symbol: BTK
Immunogen: A phospho specific peptide corresponding to residues surrounding Y223 of human BTK
Tissue Specificity Predominantly expressed in B-lymphocytes.
Post Translational Modifications Following B-cell receptor (BCR) engagement, translocates to the plasma membrane where it gets phosphorylated at Tyr-551 by LYN and SYK. Phosphorylation at Tyr-551 is followed by autophosphorylation of Tyr-223 which may create a docking site for a SH2 containing protein. Phosphorylation at Ser-180 by PRKCB, leads in translocation of BTK back to the cytoplasmic fraction. Phosphorylation at Ser-21 and Ser-115 creates a binding site for PIN1 at these Ser-Pro motifs, and promotes it's recruitment.
Function Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK. BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways. Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway. The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense. Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells. Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation. BTK plays also a critical role in transcription regulation. Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes. BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B. Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR. GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression. ARID3A and NFAT are other transcriptional target of BTK. BTK is required for the formation of functional ARID3A DNA-binding complexes. There is however no evidence that BTK itself binds directly to DNA. BTK has a dual role in the regulation of apoptosis.
Protein Name Tyrosine-Protein Kinase Btk
Agammaglobulinemia Tyrosine Kinase
B-Cell Progenitor Kinase
Bruton Tyrosine Kinase
Database Links Reactome: R-HSA-1236974
Reactome: R-HSA-166058
Reactome: R-HSA-2029482
Reactome: R-HSA-2424491
Reactome: R-HSA-2871809
Reactome: R-HSA-416476
Reactome: R-HSA-416482
Reactome: R-HSA-5602498
Reactome: R-HSA-5603041
Reactome: R-HSA-5663213
Reactome: R-HSA-8964315
Reactome: R-HSA-9664422
Reactome: R-HSA-983695
Cellular Localisation Cytoplasm
Cell Membrane
Peripheral Membrane Protein
In Steady State
Btk Is Predominantly Cytosolic
Following B-Cell Receptor (Bcr) Engagement By Antigen
Translocates To The Plasma Membrane Through Its Ph Domain
Plasma Membrane Localization Is A Critical Step In The Activation Of Btk
A Fraction Of Btk Also Shuttles Between The Nucleus And The Cytoplasm
And Nuclear Export Is Mediated By The Nuclear Export Receptor Crm1
Alternative Antibody Names Anti-Tyrosine-Protein Kinase Btk antibody
Anti-Agammaglobulinemia Tyrosine Kinase antibody
Anti-Atk antibody
Anti-B-Cell Progenitor Kinase antibody
Anti-Bpk antibody
Anti-Bruton Tyrosine Kinase antibody
Anti-BTK antibody
Anti-AGMX1 antibody
Anti-ATK antibody
Anti-BPK antibody

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