Anti-Phospho-Bad-S112 antibody [ARC1555] (STJ11102596)

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STJ11102596

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Host: Rabbit
Applications: WB
Reactivity: Human, Mouse
Short Description: Rabbit monoclonal anti-Phospho-Bad-S112 antibody is suitable for use in Western Blot.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Monoclonal
Clone ID: ARC1555
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
Uniprot ID: BAD_HUMAN
Gene ID: 572
Gene Symbol: BAD
Immunogen: A phospho specific peptide corresponding to residues surrounding S112 of human Bad
Tissue Specificity Expressed in a wide variety of tissues.
Post Translational Modifications Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Phosphorylation at Ser-99 by PKB/AKT1 is almost completely blocked by the apoptotic C-terminus cleavage product of PKN2 generated by caspases-3 activity during apoptosis. Methylation at Arg-94 and Arg-96 by PRMT1 inhibits Akt-mediated phosphorylation at Ser-99.
Function Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
Protein Name Bcl2-Associated Agonist Of Cell Death
Bad
Bcl-2-Binding Component 6
Bcl-2-Like Protein 8
Bcl2-L-8
Bcl-Xl/Bcl-2-Associated Death Promoter
Bcl2 Antagonist Of Cell Death
Database Links Reactome: R-HSA-111447
Reactome: R-HSA-111453
Reactome: R-HSA-193648
Reactome: R-HSA-198323
Reactome: R-HSA-5674400
Cellular Localisation Mitochondrion Outer Membrane
Cytoplasm
Colocalizes With Hif3a In The Cytoplasm
Upon Phosphorylation
Locates To The Cytoplasm
Alternative Antibody Names Anti-Bcl2-Associated Agonist Of Cell Death antibody
Anti-Bad antibody
Anti-Bcl-2-Binding Component 6 antibody
Anti-Bcl-2-Like Protein 8 antibody
Anti-Bcl2-L-8 antibody
Anti-Bcl-Xl/Bcl-2-Associated Death Promoter antibody
Anti-Bcl2 Antagonist Of Cell Death antibody
Anti-BAD antibody
Anti-BBC6 antibody
Anti-BCL2L8 antibody

Information sourced from Uniprot.org

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