| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1-Ser554 (520-569 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000WB 1:500-2000IHC 1:100-1:300ELISA 1:10000-40000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ACAP1 |
| Gene ID: |
9744 |
| Uniprot ID: |
ACAP1_HUMAN |
| Immunogen Region: |
520-569 aa |
| Specificity: |
Phospho-ACAP1 (S554) Polyclonal Antibody detects endogenous levels of ACAP1 protein only when phosphorylated at S554. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human Centaurin-beta1 around the phosphorylation site of Ser554 at the amino acid range 520-569 |
| Post Translational Modifications | Phosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration. |
| Function | GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration. |
| Protein Name | Arf-Gap With Coiled-Coil - Ank Repeat And Ph Domain-Containing Protein 1Centaurin-Beta-1Cnt-B1 |
| Cellular Localisation | Recycling Endosome MembranePeripheral Membrane ProteinCytoplasmic Side |
| Alternative Antibody Names | Anti-Arf-Gap With Coiled-Coil - Ank Repeat And Ph Domain-Containing Protein 1 antibodyAnti-Centaurin-Beta-1 antibodyAnti-Cnt-B1 antibodyAnti-ACAP1 antibodyAnti-CENTB1 antibodyAnti-KIAA0050 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
