• Western blot analysis of extracts of mouse testis, using PHF7 antibody (STJ110517) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PHF7 antibody (1-381) (STJ110517)

SKU:
STJ110517

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PHF7 (1-381) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PHF7
Gene ID: 51533
Uniprot ID: PHF7_HUMAN
Immunogen Region: 1-381
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-381 of human PHF7 (NP_057567.3).
Immunogen Sequence: MKTVKEKKECQRLRKSAKTR RVTQRKPSSGPVCWLCLREP GDPEKLGEFLQKDNISVHYF CLILSSKLPQRGQSNRGFHG FLPEDIKKEAARASRKICFV CKKKGAAINCQKDQCLRNFH LPCGQERGCLSQFFGEYKSF CDKHRPTQNIQHGHVGEESC ILCCEDLSQQSVENIQSPCC SQAIYHRKCIQKYAHTSAKH FFKCPQCNNRKEFPQEMLRM GIHIPDRDAAWELEPGAFS
Tissue Specificity Highly expressed in Sertoli cells, but not in germ cells in adult testis. Expression in embryonic testis is 30-times lower. Highly expressed in colon, spleen, white blood cells, pancreas, lung, liver, placenta and brain. Detected at lower levels in thymus, small intestine, ovary and kidney.
Function May play a role in spermatogenesis.
Protein Name Phd Finger Protein 7
Testis Development Protein Nyd-Sp6
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Phd Finger Protein 7 antibody
Anti-Testis Development Protein Nyd-Sp6 antibody
Anti-PHF7 antibody

Information sourced from Uniprot.org

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