• Western blot analysis of extracts of various cell lines, using PGP9. 5/UCHL1/UCHL1 antibody (STJ11101638) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.
  • Immunohistochemistry of paraffin-embedded mouse brain using PGP9. 5/UCHL1/UCHL1 Rabbit mAb (STJ11101638) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded rat brain using PGP9. 5/UCHL1/UCHL1 Rabbit mAb (STJ11101638) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human oophoroma using PGP9. 5/UCHL1/UCHL1 Rabbit mAb (STJ11101638) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of mouse brain using PGP9. 5/UCHL1/UCHL1 Rabbit mAb (STJ11101638) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PGP9.5-antibody [ARC0390] (STJ11101638)

SKU:
STJ11101638

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Host: Rabbit
Applications: WB, IHC, IF
Reactivity: Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit monoclonal antibody anti-PGP9.5 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Clonality: Monoclonal
Clone ID: ARC0390
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:50-1:200
IF 1:50-1:200
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Gene Symbol: UCHL1
Gene ID: 7345
Uniprot ID: UCHL1_HUMAN
Immunogen: A synthesized peptide derived from human PGP9.5
Tissue Specificity Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
Post Translational Modifications O-glycosylated.
Function Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins (Probable). This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
Protein Name Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1
Uch-L1
Neuron Cytoplasmic Protein 9.5
Pgp 9.5
Pgp9.5
Ubiquitin Thioesterase L1
Database Links Reactome: R-HSA-5689603
Cellular Localisation Cytoplasm
Endoplasmic Reticulum Membrane
Lipid-Anchor
About 30% Of Total Uchl1 Is Associated With Membranes In Brain
Alternative Antibody Names Anti-Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1 antibody
Anti-Uch-L1 antibody
Anti-Neuron Cytoplasmic Protein 9.5 antibody
Anti-Pgp 9.5 antibody
Anti-Pgp9.5 antibody
Anti-Ubiquitin Thioesterase L1 antibody
Anti-UCHL1 antibody

Information sourced from Uniprot.org

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