| Host: |
Rabbit |
| Applications: |
IF |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PFKL (601-780) is suitable for use in Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
IF 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PFKL |
| Gene ID: |
5211 |
| Uniprot ID: |
PFKAL_HUMAN |
| Immunogen Region: |
601-780 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 601-780 of human PFKL (NP_002617.3). |
| Immunogen Sequence: |
IHDLKVNVEHMTEKMKTDIQ RGLVLRNEKCHDYYTTEFLY NLYSSEGKGVFDCRTNVLGH LQQGGAPTPFDRNYGTKLGV KAMLWLSEKLREVYRKGRVF ANAPDSACVIGLKKKAVAFS PVTELKKDTDFEHRMPREQW WLSLRLMLKMLAQYRISMAA YVSGELEHVTRRTLSMDKGF |
| Post Translational Modifications | GlcNAcylation at Ser-529 by OGT decreases enzyme activity, leading to redirect glucose flux through the oxidative pentose phosphate pathway. Glycosylation is stimulated by both hypoxia and glucose deprivation. |
| Function | Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway. |
| Protein Name | Atp-Dependent 6-Phosphofructokinase - Liver TypeAtp-PfkPfk-L6-Phosphofructokinase Type BPhosphofructo-1-Kinase Isozyme BPfk-BPhosphohexokinase |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-70171 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Atp-Dependent 6-Phosphofructokinase - Liver Type antibodyAnti-Atp-Pfk antibodyAnti-Pfk-L antibodyAnti-6-Phosphofructokinase Type B antibodyAnti-Phosphofructo-1-Kinase Isozyme B antibodyAnti-Pfk-B antibodyAnti-Phosphohexokinase antibodyAnti-PFKL antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
