• Western blot analysis of lysates from HeLa and HepG2 cells, using E2AK3 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of HepG2 cells, using E2AK3 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of PERK Polyclonal Antibody, using Hela, HepG2, SW480, A549 cell, 4°C over night, secondary antibody (cat: (NA was diluted at 1:10000, 37°C 1hour.
  • Western blot analysis of various cells using PERK Polyclonal Antibody diluted at 1:2000
  • Immunofluorescence analysis of Hela cell. 1, PERK Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-PERK antibody (STJ95032)

SKU:
STJ95032

Current Stock:
Host: Rabbit
Applications: WB, IHC-P, IF, ELISA
Reactivity: Human
Short Description: Rabbit polyclonal anti-PERK antibody is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Specificity: PERK polyclonal antibody detects endogenous levels of PERK protein.
Uniprot ID: E2AK3_HUMAN
Gene ID: 9451
Gene Symbol: EIF2AK3
Immunogen: Synthesized peptide derived from human PERK
Immunogen Region: 30-110 aa, N-terminal
Post Translational Modifications Oligomerization of the N-terminal ER luminal domain by ER stress promotes PERK trans-autophosphorylation of the C-terminal cytoplasmic kinase domain at multiple residues including Thr-982 on the kinase activation loop. Autophosphorylated. Phosphorylated at Tyr-619 following endoplasmic reticulum stress, leading to activate its tyrosine-protein kinase activity. Dephosphorylated by PTPN1/TP1B, leading to inactivate its enzyme activity. N-glycosylated. ADP-ribosylated by PARP16 upon ER stress, which increases kinase activity.
Function Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress conditions. Key activator of the integrated stress response (ISR) required for adaptation to various stress, such as unfolded protein response (UPR) and low amino acid availability. EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.
Protein Name Eukaryotic Translation Initiation Factor 2-Alpha Kinase 3
Prkr-Like Endoplasmic Reticulum Kinase
Pancreatic Eif2-Alpha Kinase
Hspek
Database Links Reactome: R-HSA-381042
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Eukaryotic Translation Initiation Factor 2-Alpha Kinase 3 antibody
Anti-Prkr-Like Endoplasmic Reticulum Kinase antibody
Anti-Pancreatic Eif2-Alpha Kinase antibody
Anti-Hspek antibody
Anti-EIF2AK3 antibody
Anti-PEK antibody
Anti-PERK antibody

Information sourced from Uniprot.org

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