• Confocal imaging of paraffin-embedded Rat brown fat tissue using Perilipin A Rabbit monoclonal antibody (STJ11102149, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry analysis of Perilipin A in paraffin-embedded mouse brown adipose using Perilipin A Rabbit monoclonal antibody (STJ11102149) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Perilipin A in paraffin-embedded rat brown adipose using Perilipin A Rabbit monoclonal antibody (STJ11102149) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Perilipin A Rabbit monoclonal antibody (STJ11102149) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-PLIN1 antibody (400-500) [S9MR] (STJ11102149)

SKU:
STJ11102149

£212.50 - £501.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Perilipin A (400-500) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLIN1
Gene ID: 5346
Uniprot ID: PLIN1_HUMAN
Immunogen Region: 400-500
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 400-500 of human Perilipin A (O60240).
Immunogen Sequence: HYVPLPRLSLMEPESEFRDI DNPPAEVERREAERRASGAP SAGPEPAPRLAQPRRSLRSA QSPGAPPGPGLEDEVATPAA PRPGFPAVPREKPKRRVSDS F
Tissue Specificity Detected in adipocytes from white adipose tissue (at protein level). Detected in visceral adipose tissue and mammary gland.
Post Translational Modifications Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet.
Function Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. Plays a role in unilocular lipid droplet formation by activating CIDEC. Their interaction promotes lipid droplet enlargement and directional net neutral lipid transfer. May modulate lipolysis and triglyceride levels.
Protein Name Perilipin-1
Lipid Droplet-Associated Protein
Database Links Reactome: R-HSA-163560
Reactome: R-HSA-381340
Reactome: R-HSA-9031528
Cellular Localisation Endoplasmic Reticulum
Lipid Droplet
Lipid Droplet Surface-Associated
Alternative Antibody Names Anti-Perilipin-1 antibody
Anti-Lipid Droplet-Associated Protein antibody
Anti-PLIN1 antibody
Anti-PERI antibody
Anti-PLIN antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance