• Formalin-fixed, paraffin-embedded colon adenocarcinoma stained with anti-PCNA antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining of tumor cells
  • Formalin-fixed, paraffin-embedded colon adenocarcinoma stained with anti-PCNA antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining of tumor cells

Anti-PCNA antibody (STJ180215)

SKU:
STJ180215
Current Stock:
Host: Mouse
Applications: IHC-p
Reactivity: Human
Short Description: Mouse monoclonal primary antibody against PCNA.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Monoclonal
Clone ID: PC10
Conjugation: Unconjugated
Formulation: Purified antibody diluted in Tris‐HCI buffer containing stabilizing protein and <0.1% sodium azide.
Storage Instruction: Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C.
Isotype: IgG2a Kappa
Dilution Range: 1:100‐200
Gene ID: 5111
Immunogen: Recombinant rat PCNA protein
Background Expression of proliferating cell nuclear antigen (PCNA) or cyclin or polymerase delta auxiliary protein is elevated in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S‐phase and declining during G2 and M phases. Its level correlates directly with rates of cellular proliferation and DNA synthesis. PCNA/cyclin may act as an auxiliary protein of DNA polymerase‐delta to play a fundamental role in the initiation of cell proliferation.
Database Links Reactome: R-HSA-110312
Cellular Localisation Nucleus
Colocalizes With Crebbp
Ep300 And Pold1 To Sites Of Dna Damage
Forms Nuclear Foci Representing Sites Of Ongoing Dna Replication And Vary In Morphology And Number During S Phase
Together With Apex2
Is Redistributed In Discrete Nuclear Foci In Presence Of Oxidative Dna Damaging Agents
Protein Name Proliferating Cell Nuclear Antigen
Pcna
Cyclin
Positive Control Tonsil, lymph node or small intestine.

Information sourced from Uniprot.org

6 months standard