||Bovine, Human, Mouse, Rat
||FOR RESEARCH USE ONLY (RUO).
||Mouse monoclonal antibody anti-Park7/DJ-1 is suitable for use in Western Blot and Immunohistochemistry research applications.
||100 ul in PBS + 10 mM Sodium Azide.
||This antibody was protein g purified culture from supernatant.
||WB 1:2, 000IF 1:500
||Store at-20°C, and avoid repeated freeze-thaw cycles.
||Full length recombinant human Park7 expressed in and purification from E. coli.
| Tissue Specificity || Ubiquitous. Detected on epididymal sperm. Highly expressed in testis and prostate. Detected at lower levels in heart, lung, brain, liver, kidney, seminal vesicle, caput and corpus epididymis. |
| Post Translational Modifications || Sumoylated on Lys-130 by PIAS2 or PIAS4.which is essential for cell-growth promoting activity and transforming activity. Undergoes cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. |
| Function || Protein and nucleotide deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins or nucleotides and reactive carbonyl groups of glyoxals. Thus, functions as a protein deglycase that repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of advanced glycation endproducts (AGE) that cause irreversible damage. Also functions as a nucleotide deglycase able to repair glycated guanine in the free nucleotide pool (GTP, GDP, GMP, dGTP) and in DNA and RNA. Is thus involved in a major nucleotide repair system named guanine glycation repair (GG repair), dedicated to reversing methylglyoxal and glyoxal damage via nucleotide sanitization and direct nucleic acid repair. Also displays an apparent glyoxalase activity that in fact reflects its deglycase activity. Plays an important role in cell protection against oxidative stress and cell death acting as oxidative stress sensor and redox-sensitive chaperone and protease.functions probably related to its primary function. It is involved in neuroprotective mechanisms like the stabilization of NFE2L2 and PINK1 proteins, male fertility as a positive regulator of androgen signaling pathway as well as cell growth and transformation through, for instance, the modulation of NF-kappa-B signaling pathway. Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. Required for correct mitochondrial morphology and function as well as for autophagy of dysfunctional mitochondria. Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. Regulates astrocyte inflammatory responses, may modulate lipid rafts-dependent endocytosis in astrocytes and neuronal cells. In pancreatic islets, involved in the maintenance of mitochondrial reactive oxygen species (ROS) levels and glucose homeostasis in an age- and diet dependent manner. Protects pancreatic beta cells from cell death induced by inflammatory and cytotoxic setting. Binds to a number of mRNAs containing multiple copies of GG or CC motifs and partially inhibits their translation but dissociates following oxidative stress. Metal-binding protein able to bind copper as well as toxic mercury ions, enhances the cell protection mechanism against induced metal toxicity. In macrophages, interacts with the NADPH oxidase subunit NCF1 to direct NADPH oxidase-dependent ROS production, and protects against sepsis. |
| Protein Name || Parkinson Disease Protein 7 HomologContraception-Associated Protein 1Protein Cap1Fertility Protein Sp22Maillard DeglycaseParkinsonism-Associated DeglycaseProtein Dj-1Dj-1Protein/Nucleic Acid Deglycase Dj-1 |
| Database Links || Reactome: R-RNO-3899300Reactome: R-RNO-9646399 |
| Cellular Localisation || Cell MembraneLipid-AnchorCytoplasmMembrane RaftNucleusMitochondrionEndoplasmic ReticulumUnder Normal ConditionsLocated Predominantly In The Cytoplasm AndTo A Lesser ExtentIn The Nucleus And MitochondrionTranslocates To The Mitochondrion And Subsequently To The Nucleus In Response To Oxidative Stress And Exerts An Increased Cytoprotective Effect Against Oxidative DamageMembrane Raft Localization In Astrocytes And Neuronal Cells Requires Palmitoylation |
| Alternative Antibody Names || Anti-Parkinson Disease Protein 7 Homolog antibodyAnti-Contraception-Associated Protein 1 antibodyAnti-Protein Cap1 antibodyAnti-Fertility Protein Sp22 antibodyAnti-Maillard Deglycase antibodyAnti-Parkinsonism-Associated Deglycase antibodyAnti-Protein Dj-1 antibodyAnti-Dj-1 antibodyAnti-Protein/Nucleic Acid Deglycase Dj-1 antibodyAnti-Park7 antibodyAnti-Cap1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance