• Immunoprecipitation analysis of 200 Mu g extracts of A-549 cells using 3 Mu g PALLD antibody (STJ114240). Western blot was performed from the immunoprecipitate using PALLD antibody (STJ114240) at a dilution of 1:1000.
  • Immunofluorescence analysis of NIH-3T3 cells using PALLD Rabbit polyclonal antibody (STJ114240) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using PALLD Rabbit polyclonal antibody (STJ114240) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of PALLD in paraffin-embedded human colon carcinoma using PALLD Rabbit polyclonal antibody (STJ114240) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PALLD in paraffin-embedded rat ovary using PALLD Rabbit polyclonal antibody (STJ114240) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using PALLD Rabbit polyclonal antibody (STJ114240) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PALLD antibody (383-672) (STJ114240)

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STJ114240

$228.83 - $606.83
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Host: Rabbit
Applications: WB/IP/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PALLD (383-672) is suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IP 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PALLD
Gene ID: 23022
Uniprot ID: PALLD_HUMAN
Immunogen Region: 383-672
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 383-672 of human PALLD (NP_001159582.1).
Immunogen Sequence: AVNQRGRSPRSPSGHPHVRR PRSRSRDSGDENEPIQERFF RPHFLQAPGDLTVQEGKLCR MDCKVSGLPTPDLSWQLDGK PVRPDSAHKMLVRENGVHSL IIEPVTSRDAGIYTCIATNR AGQNSFSLELVVAAKEAHKP PVFIEKLQNTGVADGYPVRL ECRVLGVPPPQIFWKKENES LTHSTDRVSMHQDNHGYICL LIQGATKEDAGWYTVSAKNE AGIVSCTARLDVYTQWHQQ
Tissue Specificity Detected in both muscle and non-muscle tissues. High expression in prostate, ovary, colon, and kidney. Not detected in spleen, skeletal muscle, lung and peripheral blood lymphocytes (at protein level). Protein is overexpressed in FA6, HPAF, IMIM-PC2, SUIT-2 and PancTu-II sporadic pancreatic cancer cell lines.
Post Translational Modifications Phosphorylated predominantly on serines and, to a lesser extent, on tyrosines. Phosphorylation at Ser-1118 by PKB/AKT1 modulates cytoskeletal organization and cell motility.
Function Cytoskeletal protein required for organization of normal actin cytoskeleton. Roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. May function as a scaffolding molecule with the potential to influence both actin polymerization and the assembly of existing actin filaments into higher-order arrays. Binds to proteins that bind to either monomeric or filamentous actin. Localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Different isoforms may have functional differences. Involved in the control of morphological and cytoskeletal changes associated with dendritic cell maturation. Involved in targeting ACTN to specific subcellular foci.
Protein Name Palladin
Sih002
Sarcoma Antigen Ny-Sar-77
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Junction
Focal Adhesion
Myofibril
Sarcomere
Z Line
Cell Projection
Ruffle
Podosome
Lamellipodium
Axon
Growth Cone
Localizes To Stress Fibers And Z Lines
Preferentially Expressed In The Excitatory Presynaptic Terminals
Alternative Antibody Names Anti-Palladin antibody
Anti-Sih002 antibody
Anti-Sarcoma Antigen Ny-Sar-77 antibody
Anti-PALLD antibody
Anti-KIAA0992 antibody
Anti-CGI-151 antibody

Information sourced from Uniprot.org

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