• Western blot analysis of extracts of various cell lines, using PAK2 rabbit monoclonal antibody (STJ11102101) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of C6 cells using PAK2 antibody (STJ11102101) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using PAK2 antibody (STJ11102101) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using PAK2 antibody (STJ11102101) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-PAK2 antibody [ARC1030] (STJ11102101)


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Host: Rabbit
Applications: WB, IF
Reactivity: Human, Mouse, Rat
Short Description: Rabbit monoclonal antibody anti-PAK2 is suitable for use in Western Blot and Immunofluorescence.
Clonality: Monoclonal
Clone ID: ARC1030
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF 1:50-1:200
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Gene Symbol: PAK2
Gene ID: 5062
Uniprot ID: PAK2_HUMAN
Immunogen: A synthesized peptide derived from human PAK2
Tissue Specificity Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.
Post Translational Modifications Full-length PAK2 is autophosphorylated when activated by CDC42/p21. Following cleavage, both peptides, PAK-2p27 and PAK-2p34, become highly autophosphorylated, with PAK-2p27 being phosphorylated on serine and PAK-2p34 on threonine residues, respectively. Autophosphorylation of PAK-2p27 can occur in the absence of any effectors and is dependent on phosphorylation of Thr-402, because PAK-2p27 is acting as an exogenous substrate. During apoptosis proteolytically cleaved by caspase-3 or caspase-3-like proteases to yield active PAK-2p34. Ubiquitinated, leading to its proteasomal degradation. PAK-2p34 is myristoylated.
Function Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Phosphorylates JUN and plays an important role in EGF-induced cell proliferation. Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP. Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis. On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway. Caspase-activated PAK2 phosphorylates MKNK1 and reduces cellular translation.
Protein Name Serine/Threonine-Protein Kinase Pak 2
S6/H4 Kinase
P21-Activated Kinase 2
P58 Cleaved Into - Pak-2p27
P27 - Pak-2p34
Database Links Reactome: R-HSA-164944
Reactome: R-HSA-202433
Reactome: R-HSA-211728
Reactome: R-HSA-211733
Reactome: R-HSA-211736
Reactome: R-HSA-2871796
Reactome: R-HSA-389359
Reactome: R-HSA-3928664
Reactome: R-HSA-399954
Reactome: R-HSA-428540
Reactome: R-HSA-4420097
Reactome: R-HSA-445355
Reactome: R-HSA-5218920
Reactome: R-HSA-5621575
Reactome: R-HSA-5627123
Reactome: R-HSA-5687128
Reactome: R-HSA-8950505
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013406
Reactome: R-HSA-9013407
Reactome: R-HSA-9013408
Reactome: R-HSA-9013409
Reactome: R-HSA-9013420
Reactome: R-HSA-9013423
Reactome: R-HSA-9013424
Cellular Localisation Serine/Threonine-Protein Kinase Pak 2: Cytoplasm
Myo18a Mediates The Cellular Distribution Of The Pak2-Arhgef7-Git1 Complex To The Inner Surface Of The Cell Membrane
Pak-2p34: Nucleus
Perinuclear Region
Interaction With Arhgap10 Probably Changes Pak-2p34 Location To Cytoplasmic Perinuclear Region
Myristoylation Changes Pak-2p34 Location To The Membrane
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Pak 2 antibody
Anti-Gamma-Pak antibody
Anti-Pak65 antibody
Anti-S6/H4 Kinase antibody
Anti-P21-Activated Kinase 2 antibody
Anti-Pak-2 antibody
Anti-P58 Cleaved Into - Pak-2p27 antibody
Anti-P27 - Pak-2p34 antibody
Anti-P34 antibody
Anti-C-T-Pak2 antibody
Anti-PAK2 antibody

Information sourced from Uniprot.org

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