• STJ70617 (0.3µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • HEK293 overexpressing PADI4 (RC206501) and probed with STJ70617 (mock transfection in first lane), tested by Origene.
  • STJ70617 (0.3µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • HEK293 overexpressing PADI4 (RC206501) and probed with STJ70617 (mock transfection in first lane), tested by Origene.

Anti-PAD4/PADI4 Antibody (STJ70617)

SKU:
STJ70617

Current Stock:
Host: Goat
Applications: Pep-ELISA, WB, IHC
Reactivity: Human
Short Description: Goat polyclonal anti-PAD4/PADI4 antibody is suitable for use in ELISA, Western Blot and Immunohistochemistry.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at-20°C. Minimize freezing and thawing.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage Instruction: Store at-20°C and avoid freeze-thaw cycles.
Concentration: 0.5mg/mL
Isotype: IgG
Dilution Range: WB-0.3-1µg/ml
IHC-5µg/ml
ELISA-antibody detection limit dilution 1:64000.
Uniprot ID: PADI4_HUMAN
Gene ID: 23569
Gene Symbol: PADI4
Immunogen Region: N Terminus 663 aa
Immunogen Sequence: AQGTLIRVTPEQPC
Accession Number: NP_036519.2
Post Translational Modifications Autocitrullination at Arg-372 and Arg-374 inactivates the enzyme.
Function Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.
Protein Name Protein-Arginine Deiminase Type-4
Hl-60 Pad
Peptidylarginine Deiminase Iv
Protein-Arginine Deiminase Type Iv
Database Links Reactome: R-HSA-3247509
Cellular Localisation Cytoplasm
Nucleus
Cytoplasmic Granule
Cytoplasmic Granules Of Eosinophils And Neutrophils
Alternative Antibody Names Anti-Protein-Arginine Deiminase Type-4 antibody
Anti-Hl-60 Pad antibody
Anti-Peptidylarginine Deiminase Iv antibody
Anti-Protein-Arginine Deiminase Type Iv antibody
Anti-PADI4 antibody
Anti-PAD4 antibody
Anti-PADI5 antibody
Anti-PDI5 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance