• STJ72755 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
  • STJ72755 (0.3µg/ml) staining of A549 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-P2rx4 (mouse) Antibody (STJ72755)

SKU:
STJ72755
Current Stock:
Host: Goat
Applications: Pep-ELISA, WB, IF, IHC
Reactivity: Human, Mouse, Rat, Dog, Pig, Cow
Short Description: Goat polyclonal primary antibody against P2rx4 (mouse).
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at-20°C. Minimize freezing and thawing.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage Instruction: Store at-20° and avoid freeze-thaw cycles.
Concentration: 0.5mg/mL
Isotype: IgG
Dilution Range: WB-0.3-1µg/ml
IHC-5µg/ml
IF-Strong expression of the protein seen in the cytoplasm of A431 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:16000.
Uniprot ID: P2RX4_RAT
Gene ID: 29659
Gene Symbol: P2rx4
Immunogen Region: internal region
Immunogen Sequence: ETDSVVSSVTTKAK
Accession Number: NP_035156.2
6 months standard