Anti-ORMDL3 antibody (98-148 aa) (STJ196029)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human ORML3 at the amino acid range 98-148
General Information
| Short Description | Rabbit polyclonal anti-ORM1-like protein 3 (98-148 aa) for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ORMDL3 |
| Gene ID | 94103 |
| Uniprot ID | ORML3_HUMAN |
| Immunogen | Synthesized peptide derived from the human ORML3 at the amino acid range 98-148 |
| Immunogen Region | 98-148 aa |
| Specificity | This antibody detects endogenous levels of ORML3 at Human/Mouse/Rat |
Additional Info
| Post Translational Modifications | When hydroxylated at Pro-137, ubiquitinated via 'Lys-48'-linkage, leading to proteasomal degradation. In endothelial cells, ORMDL3 proteasomal degradation is controlled by the sphingosine 1-phosphate receptor signaling pathway. |
| Function | Plays an essential role in the homeostatic regulation of sphingolipid de novo biosynthesis by modulating the activity of the serine palmitoyltransferase (SPT) in response to ceramide levels. When complexed to SPT, the binding of ceramides to its N-terminus stabilizes a conformation that block SPT substrate entry, hence preventing SPT catalytic activity. Through this mechanism, maintains ceramide levels at sufficient concentrations for the production of complex sphingolipids, but which prevents the accumulation of ceramides to levels that trigger apoptosis. |
| Protein Name | Orm1-Like Protein 3 |
| Database Links | Reactome: R-HSA-1660661Reactome: R-HSA-6798695 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Orm1-Like Protein 3 antibodyAnti-ORMDL3 antibody |
Information sourced from Uniprot.org