Anti-NF-Kappa B p65 antibody [SM0435] (STJA0010435)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG
ImmunogenKLH conjugated Synthetic peptide corresponding to Human NF-Kappa B p65 around the phosphorylation site of Serine 536
General Information
| Short Description | Mouse monoclonal anti-NF-Kappa B p65 for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SM0435 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | WB (H, M, R) 1:500-1:1000 |
| Formulation | PBS with 0.02% sodium azide, 100 Mu g/ml BSA and 50% glycerol. |
| Storage Instruction | Store at-20C for up to one year, and avoid repeated freeze-thaw cycles. |
Target Information
| Gene Symbol | RELA |
| Gene ID | 5970 |
| Uniprot ID | TF65_HUMAN |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Human NF-Kappa B p65 around the phosphorylation site of Serine 536 |
Additional Info
| Function | NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric RELA-NFKB1 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. The NF-kappa-B heterodimeric RELA-NFKB1 and RELA-REL complexes, for instance, function as transcriptional activators. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The inhibitory effect of I-kappa-B on NF-kappa-B through retention in the cytoplasm is exerted primarily through the interaction with RELA. RELA shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Besides its activity as a direct transcriptional activator, it is also able to modulate promoters accessibility to transcription factors and thereby indirectly regulate gene expression. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells. The NF-kappa-B homodimeric RELA-RELA complex appears to be involved in invasin-mediated activation of IL-8 expression. Key transcription factor regulating the IFN response during SARS-CoV-2 infection. |
| Protein Name | Transcription Factor P65Nuclear Factor Nf-Kappa-B P65 SubunitNuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3 |
| Database Links | Reactome: R-HSA-1169091Reactome: R-HSA-1810476Reactome: R-HSA-193692Reactome: R-HSA-202424Reactome: R-HSA-209560Reactome: R-HSA-2559582Reactome: R-HSA-2871837Reactome: R-HSA-3134963Reactome: R-HSA-3214841Reactome: R-HSA-381340Reactome: R-HSA-445989Reactome: R-HSA-448706Reactome: R-HSA-4755510Reactome: R-HSA-5603029Reactome: R-HSA-5607761Reactome: R-HSA-5607764Reactome: R-HSA-5621575Reactome: R-HSA-5660668Reactome: R-HSA-844456Reactome: R-HSA-8853884Reactome: R-HSA-9020702Reactome: R-HSA-933542Reactome: R-HSA-9660826Reactome: R-HSA-9692916Reactome: R-HSA-9818749Reactome: R-HSA-9860927 |
| Cellular Localisation | NucleusCytoplasmNuclearBut Also Found In The Cytoplasm In An Inactive Form Complexed To An Inhibitor (I-Kappa-B)Colocalized With Ddx1 In The Nucleus Upon Tnf-Alpha InductionColocalizes With Gfi1 In The Nucleus After Lps StimulationTranslocation To The Nucleus Is Impaired In LMonocytogenes Infection |
| Alternative Antibody Names | Anti-Transcription Factor P65 antibodyAnti-Nuclear Factor Nf-Kappa-B P65 Subunit antibodyAnti-Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3 antibodyAnti-RELA antibodyAnti-NFKB3 antibody |
Information sourced from Uniprot.org