• Human colon adenocarcinoma with Lynch syndrome stained with anti-MSH-2 antibody using peroxidase-conjugate and DAB chromogen. Note absence of nuclear staining of tumor cells whereas the background cells are positive.

Anti-MSH2 antibody [G219‐1129] (STJ180177)

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STJ180177

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Host: Mouse
Applications: IHC-P
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-MSH2 is suitable for use in Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: G219‐1129
Conjugation: Unconjugated
Isotype: IgG1/Kappa
Formulation: Tris-HCI buffer containing stabilizing protein (BSA) and <0.1% ProClin
Purification: Affinity purified
Dilution Range: 1:50‐100
Storage Instruction: Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C.
Gene Symbol: MSH2
Gene ID: 4436
Uniprot ID: MSH2_HUMAN
Specificity: Positive control: Colon carcinoma
Immunogen: BALB/C mice were injected with recombinant human MSH2 protein
Tissue Specificity Ubiquitously expressed.
Post Translational Modifications Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.
Function Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containing DNA strand. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
Protein Name Dna Mismatch Repair Protein Msh2
Hmsh2
Muts Protein Homolog 2
Database Links Reactome: R-HSA-5358565
Reactome: R-HSA-5358606
Reactome: R-HSA-5632927
Reactome: R-HSA-5632928
Reactome: R-HSA-5632968
Reactome: R-HSA-6796648
Cellular Localisation Nucleus
Chromosome
Alternative Antibody Names Anti-Dna Mismatch Repair Protein Msh2 antibody
Anti-Hmsh2 antibody
Anti-Muts Protein Homolog 2 antibody
Anti-MSH2 antibody

Information sourced from Uniprot.org

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