• Formalin-fixed, paraffin-embedded human colon adenocarcinoma stained with anti-MSH-2 antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining of tumor cells
  • Formalin-fixed, paraffin-embedded human colon adenocarcinoma stained with anti-MSH-2 antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining of tumor cells

Anti-MSH2 antibody (STJ180177)

SKU:
STJ180177
Current Stock:
Host: Mouse
Applications: IHC-p
Reactivity: Human
Short Description: Mouse monoclonal primary antibody against MSH2.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Monoclonal
Clone ID: G219‐1129
Conjugation: Unconjugated
Formulation: Purified antibody diluted in Tris‐HCI buffer containing stabilizing protein and <0.1% sodium azide.
Storage Instruction: Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C.
Isotype: IgG1 Kappa
Dilution Range: 1:50‐100
Uniprot ID: MSH2_HUMAN
Gene ID: 4436
Gene Symbol: MSH2
Immunogen: BALB/C mice were injected with recombinant human MSH2 protein
Background Germline mutations in human mismatch repair genes (hMSH2, hMSH6, hMLH1, hPMS2) account for majority of the hereditary non‐polyposis colorectal carcinoma (HPNCC). CpG dinucleotides in the hMSH2 and hMLH1 genes are hotspots for HNPCC mutations. These mutations cause a mismatch repair deficiency resulting in a mutator phenotype where the replication errors are not repaired. Microsatellites/simple repetitive sequences are prone to this type of replication errors and instability of these microsatellites correlates with the occurrence of HPNCC. hMSH2 binds to another MutS homolog protein GTBP to form a heterodimeric complex called hMutS beta, which binds to insertion/deletion loops in DNA.
Tissue Specificity Ubiquitously expressed.
Post Translational Modifications Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.
Database Links Reactome: R-HSA-5358565
Cellular Localisation Nucleus
Chromosome
Protein Name Dna Mismatch Repair Protein Msh2
Hmsh2
Muts Protein Homolog 2
Alternative Names Anti-Dna Mismatch Repair Protein Msh2 antibody
Anti-Hmsh2 antibody
Anti-Muts Protein Homolog 2 antibody
Anti-MSH2 antibody
Positive Control Colon carcinoma.

Information sourced from Uniprot.org

6 months standard