• Western blot analysis of 1) Hela, 2) 3T3, diluted at 1:2000.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Mono-Methyl-Histone H2B-Lys5 antibody (STJ97162)

SKU:
STJ97162

Current Stock:
Host: Mouse
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse polyclonal antibody anti-Mono-Methyl-Histone H2B type 1-A and Histone H2B type 1-B and Histone H2B type 1-C/E/F/G/I-Lys5 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: WB 1:500-1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: H2BC4.H2BC6.H2BC7.H2BC8.H2BC10
H2BC1
Gene ID: 3017/8339/8343/8344/8346/8347
255626
H2B1C_HUMAN
H2B1A_HUMAN
H2B1B_HUMAN
Specificity: The antibody detects endogenous Histone H2B (Mono Methyl Lys5) protein.
Immunogen: Synthetic Peptide of Histone H2B (Mono Methyl Lys5)
Post Translational Modifications Monoubiquitination at Lys-36 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-122 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. Acetylated during spermatogenesis. Acetylated form is most abundant in spermatogonia compared to spermatocytes and round spermatids. Phosphorylated at Thr-117 in spermatogonia, spermatocytes and round spermatids. Methylated at Lys-118 in spermatogonia, spermatocytes and round spermatids.
Function Variant histone specifically required to direct the transformation of dissociating nucleosomes to protamine in male germ cells. Entirely replaces classical histone H2B prior nucleosome to protamine transition and probably acts as a nucleosome dissociating factor that creates a more dynamic chromatin, facilitating the large-scale exchange of histones. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Also found in fat cells, its function and the presence of post-translational modifications specific to such cells are still unclear.
Protein Name Histone H2b Type 1-A
Histone H2b - Testis
Tsh2b.1
Htsh2b
Testis-Specific Histone H2b
Database Links Reactome: R-HSA-110328
Reactome: R-HSA-110329
Reactome: R-HSA-110330
Reactome: R-HSA-110331
Reactome: R-HSA-1221632
Reactome: R-HSA-171306
Reactome: R-HSA-1912408
Reactome: R-HSA-201722
Reactome: R-HSA-212300
Reactome: R-HSA-2299718
Reactome: R-HSA-2559580
Reactome: R-HSA-2559582
Reactome: R-HSA-2559586
Reactome: R-HSA-3214815
Reactome: R-HSA-3214847
Reactome: R-HSA-427359
Reactome: R-HSA-427389
Reactome: R-HSA-427413
Reactome: R-HSA-5250924
Reactome: R-HSA-5334118
Reactome: R-HSA-5578749
Reactome: R-HSA-5617472
Reactome: R-HSA-5625886
Reactome: R-HSA-5689880
Reactome: R-HSA-5693565
Reactome: R-HSA-5693571
Reactome: R-HSA-5693607
Reactome: R-HSA-606279
Reactome: R-HSA-69473
Reactome: R-HSA-73728
Reactome: R-HSA-73772
Reactome: R-HSA-8866654
Reactome: R-HSA-8936459
Reactome: R-HSA-8939236
Reactome: R-HSA-9018519
Reactome: R-HSA-912446
Reactome: R-HSA-9609690
Reactome: R-HSA-9610379
Reactome: R-HSA-9616222
Reactome: R-HSA-9670095
Reactome: R-HSA-977225
Cellular Localisation Nucleus
Chromosome
Alternative Antibody Names Anti-Histone H2b Type 1-A antibody
Anti-Histone H2b - Testis antibody
Anti-Tsh2b.1 antibody
Anti-Htsh2b antibody
Anti-Testis-Specific Histone H2b antibody
Anti-H2BC1 antibody
Anti-HIST1H2BA antibody
Anti-TSH2B antibody

Information sourced from Uniprot.org

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