• Immunohistochemistry of paraffin-embedded human lung cancer using CD3 epsilon Rabbit mAb (STJ11101504) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of Jurkat cells, using CD3 epsilon antibody (STJ11101504) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 3min.

Anti-Methyl-EEF2-H565 antibody (STJ11101504)

SKU:
STJ11101504
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Host: Rabbit
Applications: WB
Reactivity: Human
Short Description: Rabbit polyclonal primary antibody against Methyl-EEF2-H565.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
Uniprot ID: EF2_HUMAN
Gene ID: 1938
Gene Symbol: EEF2
Immunogen: Recombinant protein of human Methyl-EEF2-H565.
Post Translational Modifications Phosphorylation by EF-2 kinase completely inactivates EF-2.it requires prior phosphorylation by CDK2 at Ser-595 during mitotic prometaphase. Phosphorylation by CSK promotes SUMOylation, proteolytic cleavage, and nuclear translocation if the C-terminal fragment. Diphthamide is 2-3-carboxyamido-3-(trimethyl-ammonio)propylhistidine. (Microbial infection) Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis. ISGylated. Proteolytically processed at two sites following phosphorylation by CSK. SUMOylated following phosphorylation by CSK, promotes proteolytic cleavage.
Database Links Reactome: R-HSA-156902
Cellular Localisation Cytoplasm
Nucleus
Phosphorylation By Csk Promotes Cleavage And Sumoylation-Dependent Nuclear Translocation Of The C-Terminal Cleavage Product
Protein Name Elongation Factor 2
Ef-2
Alternative Names Anti-Elongation Factor 2 antibody
Anti-Ef-2 antibody
Anti-EEF2 antibody
Anti-EF2 antibody

Information sourced from uniprot.org

6 months standard