• Human atypical lipomatous tumor stained with anti-MDM2 antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining of tumor cells.

Anti-MDM2 antibody [SMP14] (STJ180166)

SKU:
STJ180166

Current Stock:
Host: Mouse
Applications: IHC-p
Reactivity: Human
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Mouse monoclonal antibody Anti-MDM2 is suitable for use in Immunohistochemistry.
Clonality: Monoclonal
Clone ID: SMP14
Conjugation: Unconjugated
Isotype: IgG1 Kappa
Formulation: Purified antibody diluted in Tris‐HCI buffer containing stabilizing protein and <0.1% sodium azide.
Dilution Range: 1:50‐100
Storage Instruction: Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C.
Gene Symbol: MDM2
Gene ID: 4193
Uniprot ID: MDM2_HUMAN
Specificity: Positive control tissue: Liposarcoma.
Immunogen: Amino acids 154‐167 of MDM2 of human origin
Tissue Specificity Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.
Post Translational Modifications Phosphorylation on Ser-166 by SGK1 activates ubiquitination of p53/TP53. Phosphorylated at multiple sites near the RING domain by ATM upon DNA damage.this prevents oligomerization and E3 ligase processivity and impedes constitutive p53/TP53 degradation. Autoubiquitination leads to proteasomal degradation.resulting in p53/TP53 activation it may be regulated by SFN. Also ubiquitinated by TRIM13. Deubiquitinated by USP2 leads to its accumulation and increases deubiquitination and degradation of p53/TP53. Deubiquitinated by USP7 leading to its stabilization.
Function E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation. Ubiquitinates DCX, leading to DCX degradation and reduction of the dendritic spine density of olfactory bulb granule cells. Ubiquitinates DLG4, leading to proteasomal degradation of DLG4 which is required for AMPA receptor endocytosis. Negatively regulates NDUFS1, leading to decreased mitochondrial respiration, marked oxidative stress, and commitment to the mitochondrial pathway of apoptosis. Binds NDUFS1 leading to its cytosolic retention rather than mitochondrial localization resulting in decreased supercomplex assembly (interactions between complex I and complex III), decreased complex I activity, ROS production, and apoptosis.
Protein Name E3 Ubiquitin-Protein Ligase Mdm2
Double Minute 2 Protein
Hdm2
Oncoprotein Mdm2
Ring-Type E3 Ubiquitin Transferase Mdm2
P53-Binding Protein Mdm2
Database Links Reactome: R-HSA-198323
Reactome: R-HSA-2559580
Reactome: R-HSA-2559585
Reactome: R-HSA-3232118
Reactome: R-HSA-3232142
Reactome: R-HSA-399719
Reactome: R-HSA-5674400
Reactome: R-HSA-5689880
Reactome: R-HSA-6804756
Reactome: R-HSA-6804757
Reactome: R-HSA-6804760
Reactome: R-HSA-69541
Reactome: R-HSA-8941858
Cellular Localisation Nucleus
Nucleoplasm
Cytoplasm
Nucleolus
Expressed Predominantly In The Nucleoplasm
Interaction With Arf(P14) Results In The Localization Of Both Proteins To The Nucleolus
The Nucleolar Localization Signals In Both Arf(P14) And Mdm2 May Be Necessary To Allow Efficient Nucleolar Localization Of Both Proteins
Colocalizes With Rassf1 Isoform A In The Nucleus
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Mdm2 antibody
Anti-Double Minute 2 Protein antibody
Anti-Hdm2 antibody
Anti-Oncoprotein Mdm2 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Mdm2 antibody
Anti-P53-Binding Protein Mdm2 antibody
Anti-MDM2 antibody

Information sourced from Uniprot.org

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