• WB on mouse kidney lysate. Blocking with 0.5% LFDM for 30 min at RT; Primary antibody used at 1: 1000 dilution incubated overnight at 4C.
  • IHC-P on paraffin sections of rat brain.The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
  • IHC-P on paraffin sections of rat brain.The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
  • IHC-P on paraffin sections of rat brain.The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
  • IHC-P on paraffin sections of rat brain.The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Anti-MCOLN1 antibody (150-210) (STJ13100249)

SKU:
STJ13100249

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Host: NZ White Rabbit
Applications: IHC, WB
Reactivity: Mouse, Rat, Human, Marmoset
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Nz White Rabbit polyclonal antibody anti-Mucolipin 1 (150-210) is suitable for use in Immunohistochemistry and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Shipped as lyophilised. Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Purification: Whole serum
Dilution Range: A dilution of 1:1000 to 1:3000 is recommended. The optimal dilution should be determined by the end user.
Storage Instruction: Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Gene Symbol: MCOLN1
Gene ID: 57192
Uniprot ID: MCLN1_HUMAN
Immunogen Region: 150-210
Specificity: Specific for Mucolipin 1.
Immunogen: A synthetic peptide from aa 150-210 of mouse Mucolipin 1 conjugated to blue carrier protein was used as the antigen.
Post Translational Modifications Palmitoylated.involved in association with membranes. Phosphorylation by PKA inhibits channel activity. Dephosphorylation increases activity. Proteolytically cleaved probably involving multiple lysosomal proteases including cathepsin B.inhibits lysosomal channel activity.
Function Nonselective cation channel probably playing a role in the regulation of membrane trafficking events and of metal homeostasis. Proposed to play a major role in Ca(2+) release from late endosome and lysosome vesicles to the cytoplasm, which is important for many lysosome-dependent cellular events, including the fusion and trafficking of these organelles, exocytosis and autophagy. Required for efficient uptake of large particles in macrophages in which Ca(2+) release from the lysosomes triggers lysosomal exocytosis. May also play a role in phagosome-lysosome fusion. Involved in lactosylceramide trafficking indicative for a role in the regulation of late endocytic membrane fusion/fission events. By mediating lysosomal Ca(2+) release is involved in regulation of mTORC1 signaling and in mTOR/TFEB-dependent lysosomal adaptation to environmental cues such as nutrient levels. Seems to act as lysosomal active oxygen species (ROS) sensor involved in ROS-induced TFEB activation and autophagy. Functions as a Fe(2+) permeable channel in late endosomes and lysosomes. Proposed to play a role in zinc homeostasis probably implicating its association with TMEM163 In adaptive immunity, TRPML2 and TRPML1 may play redundant roles in the function of the specialized lysosomes of B cells. May contribute to cellular lipase activity within the late endosomal pathway or at the cell surface which may be involved in processes of membrane reshaping and vesiculation, especially the growth of tubular structures. However, it is not known, whether it conveys the enzymatic activity directly, or merely facilitates the activity of an associated phospholipase.
Protein Name Mucolipin-1
Ml1
Mg-2
Mucolipidin
Transient Receptor Potential Channel Mucolipin 1
Trpml1
Database Links Reactome: R-HSA-3295583
Reactome: R-HSA-917977
Cellular Localisation Late Endosome Membrane
Multi-Pass Membrane Protein
Lysosome Membrane
Cytoplasmic Vesicle Membrane
Cell Projection
Phagocytic Cup
Cytoplasmic Vesicle
Phagosome Membrane
Cell Membrane
Delivery From The Trans-Golgi To Lysosomes Seems To Occur Mainly In A Direct Intracellular Manner Without Intermediate Delivery To The Plasma Membrane
Under Normal Conditions
Restricted To Intracellular Compartments So That Only A Very Minor Proportion Is Present At The Cell Membrane
Alternative Antibody Names Anti-Mucolipin-1 antibody
Anti-Ml1 antibody
Anti-Mg-2 antibody
Anti-Mucolipidin antibody
Anti-Transient Receptor Potential Channel Mucolipin 1 antibody
Anti-Trpml1 antibody
Anti-MCOLN1 antibody
Anti-ML4 antibody
Anti-TRPML1 antibody
Anti-MSTP080 antibody

Information sourced from Uniprot.org

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