• Immunofluorescent image of primary mouse neurons specifically labelling MAP2 (STJ120296, 1:1000, red). The blue nuclear stain is Hoechst. The primary mouse neurons were initially fixed with 4% PFA for 30 minutes, and then washed with PBS. The cells were blocked with 3% bovine serum albumin in PBS for 90 minutes before incubating with primary antibody overnight at 4C. The anti-MAP2 antibody was diluted in the blocking buffer. An Alexa-568 anti-goat secondary antibody was used at 1:500. The tissue sections were washed with PBS between each of the blocking and incubation steps.
  • Immunofluorescence of a section of rat brain stem showing specific labeling of MAP2 (STJ120296, 1:2000, red) in the perikarya and dendrites of neurons and specific labeling of the myelin sheath around axons with anti-MBP (STJ120140, 1:5000, green). The blue is DAPI revealing nuclear DNA.
  • Western blot of rat cortical lysate showing specific immunolabeling of the ~280 kDa MAP2 protein.

Anti-MAP2-MAP2 antibody (STJ120296)

SKU:
STJ120296

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Host: Goat
Applications: ICC, IHC, WB
Reactivity: Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Goat polyclonal antibody Anti-Microtubule Associated Protein 2 MAP2 is suitable for use in Immunocytochemistry, Immunohistochemistry and Western Blot.
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: 100 ul in PBS + 50% glycerol and 5 mm NaN3
Purification: This antibody was protein g purified from serum.
Dilution Range: WB 1:1, 000
IF 1:500
Storage Instruction: Store at-20°C, and avoid repeated freeze-thaw cycles.
Gene Symbol: MAP2
Gene ID: 4133
Uniprot ID: MTAP2_HUMAN
Immunogen: recombinant human MAP2 protein expressed in and purified from E. Coli
Post Translational Modifications Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly. Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation.
Function The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Protein Name Microtubule-Associated Protein 2
Map-2
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Dendrite
Alternative Antibody Names Anti-Microtubule-Associated Protein 2 antibody
Anti-Map-2 antibody
Anti-MAP2 antibody

Information sourced from Uniprot.org

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