• Immunolabeling of mouse hippocampal cultures labeled with anti-MAP2 (STJ120119, 1:1000, red) and anti-GFAP (STJ120101, 1:1000, green).
  • Immunofluorescence of a section of rat brain stem showing colabeled with Anti-MAP2 (STJ120119, red, 1:5000) and Anti-FOX3 (STJ120289, green, 1:1000). The Anti-FOX3 specifically labels the nuclei and the proximal cytoplasm of neuronal cells while the Anti-MAP2 labels dendrites and overlaps with FOX3 labeling the perikarya of neurons. The blue is DAPI staining of nuclear DNA.
  • Immunolabeling of mouse cortical cultures labeled with anti-MAP2 (STJ120119, 1:1000, red) and anti-GFAP (STJ120101, 1:1000, green).
  • Immunostaining of mixed neuron and glial cultures showing specific, strong labeling of dendrites and perikarya of neuronal cells with Anti-Microtubule Associated Protein 2 (STJ120119, red, 1:2500) and cell nuclei staining with DAPI.
  • Immunofluorescence of a section of rat brain stem showing colabeled with Anti-MAP2 (STJ120119, red, 1:5000) and Anti-FOX3 (STJ120289, green, 1:1000). The Anti-FOX3 specifically labels the nuclei and the proximal cytoplasm of neuronal cells while the Anti-MAP2 labels dendrites and overlaps with FOX3 labeling the perikarya of neurons. The blue is DAPI staining of nuclear DNA.
  • Immunofluorescence of cortical neuron-glial cell culture from E20 rat labeled with Anti-MAP2 (STJ120119, red, 1:10, 000) , and Anti-Tau (green). The blue is DAPI staining of nuclear DNA. The anti-MAP2 antibody stains dendrites and perikarya of neurons, while the anti-TAU antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green.
  • Western blot of rat cortical lysate showing specific immunolabeling of the ~280 kDa MAP2 protein.

Anti-MAP2 antibody (STJ120119)

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STJ120119
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Host: Chicken
Applications: WB, IF
Reactivity: Bovine, Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Chicken polyclonal primary antibody against MAP2.
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Total IgY fraction in PBS + 10 mM NaN3.
Purification: This antibody was total igy fraction.
Storage Instruction: Store at-20°C, and avoid repeated freeze-thaw cycles.
Dilution Range: WB 1:2, 0000
IF 1:2500
Uniprot ID: MTAP2_HUMAN
Gene ID: 4133
Gene Symbol: MAP2
Immunogen: recombinant bovine MAP2 protein expressed in and purification from E. Coli.
Background Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs) , which includes the protein Tau (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994). MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide Western blot of rat cortical lysate showing specific immunolabeling of the ~280 kDa MAP2 protein. sequences, which are very similar to those found in the related microtubule binding protein Tau (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone (Fontaine-Lenore V. et al., 2006).
Post Translational Modifications Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly. Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation.
Function The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Dendrite
Protein Name Microtubule-Associated Protein 2
Map-2
Alternative Names Anti-Microtubule-Associated Protein 2 antibody
Anti-Map-2 antibody
Anti-MAP2 antibody

Information sourced from uniprot.org

6 months standard