• Western blot analysis of extracts of various cell lines, using IRAK4 antibody (STJ27964) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 1s.
  • Immunohistochemistry of paraffin-embedded rat spleen using IRAK4 Rabbit pAb (STJ27964) at dilution of 1:200 (40x lens).
  • Immunohistochemistry of paraffin-embedded rat heart using IRAK4 Rabbit pAb (STJ27964) at dilution of 1:200 (40x lens).
  • Immunohistochemistry of paraffin-embedded rat brain using IRAK4 Rabbit pAb (STJ27964) at dilution of 1:50 (40x lens).
  • Immunohistochemistry of paraffin-embedded rat heart using IRAK4 Rabbit pAb (STJ27964) at dilution of 1:50 (40x lens).
  • Immunohistochemistry of paraffin-embedded human lung cancer using IRAK4 Rabbit pAb (STJ27964) at dilution of 1:50 (40x lens).
  • Immunohistochemistry of paraffin-embedded Human hepatobiliary duct using IRAK4 Rabbit pAb (STJ27964) at dilution of 1:50 (40x lens).

Anti-IRAK4-antibody (aa1-220) (STJ27964)

SKU:
STJ27964

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Host: Rabbit
Applications: WB, IHC, IF
Reactivity: Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-IRAK4 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:50-1:200
IF 1:50-1:200
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Gene Symbol: IRAK4
Gene ID: 51135
Uniprot ID: IRAK4_HUMAN
Immunogen Region: 1-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human IRAK4 (NP_057207.2).
Post Translational Modifications Phosphorylated.
Function Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.
Protein Name Interleukin-1 Receptor-Associated Kinase 4
Irak-4
Renal Carcinoma Antigen Ny-Ren-64
Database Links Reactome: R-HSA-1257604
Reactome: R-HSA-166058
Reactome: R-HSA-5603037
Reactome: R-HSA-5603041
Reactome: R-HSA-6811558
Reactome: R-HSA-9020702
Reactome: R-HSA-975110
Reactome: R-HSA-975138
Reactome: R-HSA-975155
Reactome: R-HSA-975871
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Interleukin-1 Receptor-Associated Kinase 4 antibody
Anti-Irak-4 antibody
Anti-Renal Carcinoma Antigen Ny-Ren-64 antibody
Anti-IRAK4 antibody

Information sourced from Uniprot.org

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