| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-IFNAR1 (28-557) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
IFNAR1 |
| Gene ID: |
3454 |
| Uniprot ID: |
INAR1_HUMAN |
| Immunogen Region: |
28-557 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 28-557 of human IFNAR1 (NP_000620.2). |
| Immunogen Sequence: |
KNLKSPQKVEVDIIDDNFIL RWNRSDESVGNVTFSFDYQK TGMDNWIKLSGCQNITSTKC NFSSLKLNVYEEIKLRIRAE KENTSSWYEVDSFTPFRKAQ IGPPEVHLEAEDKAIVIHIS PGTKDSVMWALDGLSFTYSL VIWKNSSGVEERIENIYSRH KIYKLSPETTYCLKVKAALL TSWKIGVYSPVHCIKTTVEN ELPPPENIEVSVQNQNYVLK WDYTYANMTFQVQWLHAFL |
| Tissue Specificity | IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266B1. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R. |
| Post Translational Modifications | Ubiquitinated, leading to its internalization and degradation. Polyubiquitinated via 'Lys-48'-linked and 'Lys-63'-linked ubiquitin chains, leading to receptor internalization and lysosomal degradation. The 'Lys-63'-linked ubiquitin chains are cleaved off by the BRISC complex. Phosphorylated on tyrosine residues in response to interferon-binding: phosphorylation by TYK2 tyrosine kinase creates docking sites for STAT proteins. Phosphorylated on serine residues in response to interferon binding.this promotes interaction with FBXW11 and ubiquitination. Palmitoylation at Cys-463 is required for the activation of STAT1 and STAT2. |
| Function | Together with IFNAR2, forms the heterodimeric receptor for type I interferons (including interferons alpha, beta, epsilon, omega and kappa). Type I interferon binding activates the JAK-STAT signaling cascade, resulting in transcriptional activation or repression of interferon-regulated genes that encode the effectors of the interferon response. Mechanistically, type I interferon-binding brings the IFNAR1 and IFNAR2 subunits into close proximity with one another, driving their associated Janus kinases (JAKs) (TYK2 bound to IFNAR1 and JAK1 bound to IFNAR2) to cross-phosphorylate one another. The activated kinases phosphorylate specific tyrosine residues on the intracellular domains of IFNAR1 and IFNAR2, forming docking sites for the STAT transcription factors. STAT proteins are then phosphorylated by the JAKs, promoting their translocation into the nucleus to regulate expression of interferon-regulated genes. Can also act independently of IFNAR2: form an active IFNB1 receptor by itself and activate a signaling cascade that does not involve activation of the JAK-STAT pathway. |
| Protein Name | Interferon Alpha/Beta Receptor 1Ifn-R-1Ifn-Alpha/Beta Receptor 1Cytokine Receptor Class-Ii Member 1Cytokine Receptor Family 2 Member 1Crf2-1Type I Interferon Receptor 1 |
| Database Links | Reactome: R-HSA-909733Reactome: R-HSA-912694Reactome: R-HSA-9679191Reactome: R-HSA-9705671 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinLate EndosomeLysosomeInterferon Binding Triggers Internalization Of The Receptor From The Cell Membrane Into Endosomes And Then Into Lysosomes |
| Alternative Antibody Names | Anti-Interferon Alpha/Beta Receptor 1 antibodyAnti-Ifn-R-1 antibodyAnti-Ifn-Alpha/Beta Receptor 1 antibodyAnti-Cytokine Receptor Class-Ii Member 1 antibodyAnti-Cytokine Receptor Family 2 Member 1 antibodyAnti-Crf2-1 antibodyAnti-Type I Interferon Receptor 1 antibodyAnti-IFNAR1 antibodyAnti-IFNAR antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
