• Immunofluorescence analysis of paraffin-embedded rat brain using AIF1/IBA1 Rabbit polyclonal antibody (STJ11100899) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of AIF1/IBA1 in paraffin-embedded Human brain using AIF1/IBA1 Rabbit polyclonal antibody (STJ11100899) at dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of AIF1/IBA1 in paraffin-embedded Rat brain using AIF1/IBA1 Rabbit polyclonal antibody (STJ11100899) at dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-AIF1 antibody (1-147) (STJ11100899)

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STJ11100899

£169.50 - £449.50
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Host: Rabbit
Applications: IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Iba1 (1-147) is suitable for use in Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AIF1
Gene ID: 199
Uniprot ID: AIF1_HUMAN
Immunogen Region: 1-147
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human AIF1/IBA1 (P55008).
Immunogen Sequence: MSQTRDLQGGKAFGLLKAQQ EERLDEINKQFLDDPKYSSD EDLPSKLEGFKEKYMEFDLN GNGDIDIMSLKRMLEKLGVP KTHLELKKLIGEVSSGSGET FSYPDFLRMMLGKRSAILKM ILMYEEKAREKEKPTGPPAK KAISELP
Tissue Specificity Detected in T-lymphocytes and peripheral blood mononuclear cells.
Post Translational Modifications Phosphorylated on serine residues.
Function Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
Protein Name Allograft Inflammatory Factor 1
Aif-1
Ionized Calcium-Binding Adapter Molecule 1
Protein G1
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Ruffle Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Phagocytic Cup
Associated With The Actin Cytoskeleton At Membrane Ruffles And At Sites Of Phagocytosis
Alternative Antibody Names Anti-Allograft Inflammatory Factor 1 antibody
Anti-Aif-1 antibody
Anti-Ionized Calcium-Binding Adapter Molecule 1 antibody
Anti-Protein G1 antibody
Anti-AIF1 antibody
Anti-G1 antibody
Anti-IBA1 antibody

Information sourced from Uniprot.org

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