• STJ70909 (0.01µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • STJ70909 (0.003µg/ml) staining of Human Adrenal Gland lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • STJ70909 (0.01µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • STJ70909 (0.003µg/ml) staining of Human Adrenal Gland lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-HSD3B1 Antibody (STJ70909)

SKU:
STJ70909

Current Stock:
Host: Goat
Applications: Pep-ELISA, WB, IHC
Reactivity: Human
Short Description: Goat polyclonal anti-HSD3B1 antibody is suitable for use in ELISA, Western Blot and Immunohistochemistry.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at-20°C. Minimize freezing and thawing.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage Instruction: Store at-20°C and avoid freeze-thaw cycles.
Concentration: 0.5mg/mL
Isotype: IgG
Dilution Range: WB-0.003-0.01µg/ml
IHC-3.75µg/ml
ELISA-antibody detection limit dilution 1:128000.
Uniprot ID: 3BHS1_HUMAN
Gene ID: 3283
Gene Symbol: HSD3B1
Immunogen Region: C Terminus
Immunogen Sequence: DRHKETLKSKTQ
Accession Number: NP_000853.1
Function A bifunctional enzyme responsible for the oxidation and isomerization of 3beta-hydroxy-Delta(5)-steroid precursors to 3-oxo-Delta(4)-steroids, an essential step in steroid hormone biosynthesis. Specifically catalyzes the conversion of pregnenolone to progesterone, 17alpha-hydroxypregnenolone to 17alpha-hydroxyprogesterone, dehydroepiandrosterone (DHEA) to 4-androstenedione, and androstenediol to testosterone. Additionally, catalyzes the interconversion between 3beta-hydroxy and 3-oxo-5alpha-androstane steroids controlling the bioavalability of the active forms. Specifically converts dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Also converts androstanedione, a precursor of testosterone and estrone, to epiandrosterone. Expected to use NAD(+) as preferred electron donor for the 3beta-hydroxy-steroid dehydrogenase activity and NADPH for the 3-ketosteroid reductase activity (Probable).
Protein Name 3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type 1
3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type I
3-Beta-Hsd I
3-Beta-Hydroxy-5-Ene Steroid Dehydrogenase
3-Beta-Hydroxy-Delta(5-Steroid Dehydrogenase
3-Beta-Hydroxysteroid 3-Dehydrogenase
Delta-5-3-Ketosteroid Isomerase
Dihydrotestosterone Oxidoreductase
Steroid Delta-Isomerase
Trophoblast Antigen Fdo161g
Database Links Reactome: R-HSA-193048
Reactome: R-HSA-193993
Reactome: R-HSA-194002
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Membrane Protein
Mitochondrion Membrane
Alternative Antibody Names Anti-3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type 1 antibody
Anti-3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type I antibody
Anti-3-Beta-Hsd I antibody
Anti-3-Beta-Hydroxy-5-Ene Steroid Dehydrogenase antibody
Anti-3-Beta-Hydroxy-Delta(5-Steroid Dehydrogenase antibody
Anti-3-Beta-Hydroxysteroid 3-Dehydrogenase antibody
Anti-Delta-5-3-Ketosteroid Isomerase antibody
Anti-Dihydrotestosterone Oxidoreductase antibody
Anti-Steroid Delta-Isomerase antibody
Anti-Trophoblast Antigen Fdo161g antibody
Anti-HSD3B1 antibody
Anti-3BH antibody
Anti-HSDB3A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance