• Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-HSD3B1 antibody (30-110) (STJ190453)


Current Stock:
Host: Rabbit
Applications: WB, ELISA
Reactivity: Human, Rat, Mouse
Short Description: Rabbit polyclonal antibody anti-3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type 1 (30-110) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 50% Glycerol and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit anti-serum by affinity-chromatography.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
ELISA 1:5000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HSD3B1
Gene ID: 3283
Uniprot ID: 3BHS1_HUMAN
Immunogen Region: 30-110
Specificity: HSD3B1 polyclonal antibody (3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type 1) binds to endogenous 3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type 1 at the amino acid region 30-110.
Immunogen: Synthesized peptide derived from human protein at aa range 30-110
Function A bifunctional enzyme responsible for the oxidation and isomerization of 3beta-hydroxy-Delta(5)-steroid precursors to 3-oxo-Delta(4)-steroids, an essential step in steroid hormone biosynthesis. Specifically catalyzes the conversion of pregnenolone to progesterone, 17alpha-hydroxypregnenolone to 17alpha-hydroxyprogesterone, dehydroepiandrosterone (DHEA) to 4-androstenedione, and androstenediol to testosterone. Additionally, catalyzes the interconversion between 3beta-hydroxy and 3-oxo-5alpha-androstane steroids controlling the bioavalability of the active forms. Specifically converts dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Also converts androstanedione, a precursor of testosterone and estrone, to epiandrosterone. Expected to use NAD(+) as preferred electron donor for the 3beta-hydroxy-steroid dehydrogenase activity and NADPH for the 3-ketosteroid reductase activity (Probable).
Protein Name 3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type 1
3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type I
3-Beta-Hsd I
3-Beta-Hydroxy-5-Ene Steroid Dehydrogenase
3-Beta-Hydroxy-Delta(5-Steroid Dehydrogenase
3-Beta-Hydroxysteroid 3-Dehydrogenase
Delta-5-3-Ketosteroid Isomerase
Dihydrotestosterone Oxidoreductase
Steroid Delta-Isomerase
Trophoblast Antigen Fdo161g
Database Links Reactome: R-HSA-193048
Reactome: R-HSA-193993
Reactome: R-HSA-194002
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Membrane Protein
Mitochondrion Membrane
Alternative Antibody Names Anti-3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type 1 antibody
Anti-3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type I antibody
Anti-3-Beta-Hsd I antibody
Anti-3-Beta-Hydroxy-5-Ene Steroid Dehydrogenase antibody
Anti-3-Beta-Hydroxy-Delta(5-Steroid Dehydrogenase antibody
Anti-3-Beta-Hydroxysteroid 3-Dehydrogenase antibody
Anti-Delta-5-3-Ketosteroid Isomerase antibody
Anti-Dihydrotestosterone Oxidoreductase antibody
Anti-Steroid Delta-Isomerase antibody
Anti-Trophoblast Antigen Fdo161g antibody
Anti-HSD3B1 antibody
Anti-3BH antibody
Anti-HSDB3A antibody

Information sourced from Uniprot.org

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