• Western blot analysis of lysates from Jurkat, HepG2, COLO205, and 293 cells, using HMGB1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of huvec-uv cells using HMG-1 Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Western blot analysis of various cells using HMG-1 Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Western blot analysis of Jurkat cells using HMG-1 Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Immunofluorescence analysis of Hela cell. 1, HMG-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). p53 monoclonal antibody (6C4) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-HMG-1 antibody (STJ93548)

SKU:
STJ93548
Current Stock:
Host: Rabbit
Applications: WB, IHC-P, IF, IP, ELISA
Reactivity: Human,Mouse,Rat
Short Description: Rabbit polyclonal primary antibody against HMG-1.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Specificity: HMG-1 polyclonal antibody detects endogenous levels of HMG-1 protein.
Uniprot ID: HMGB1_HUMAN
Gene ID: 3146
Gene Symbol: HMGB1
Immunogen: Synthesized peptide derived from the Internal region of human HMG-1
Immunogen Region: 100-180 aa, Internal
Tissue Specificity Ubiquituous. Expressed in platelets.
Database Links Reactome: R-HSA-140342
Cellular Localisation Nucleus
Chromosome
Cytoplasm
Secreted
Cell Membrane
Peripheral Membrane Protein
Extracellular Side
Endosome
Endoplasmic Reticulum-Golgi Intermediate Compartment
In Basal State Predominantly Nuclear
Shuttles Between The Cytoplasm And The Nucleus
Translocates From The Nucleus To The Cytoplasm Upon Autophagy Stimulation
Release From Macrophages In The Extracellular Milieu Requires The Activation Of Nlrc4 Or Nlrp3 Inflammasomes
Passively Released To The Extracellular Milieu From Necrotic Cells By Diffusion
Involving The Fully Reduced Hgmb1 Which Subsequently Gets Oxidized
Also Released From Apoptotic Cells
Active Secretion From A Variety Of Immune And Non-Immune Cells Such As Macrophages
Monocytes
Neutrophils
Dendritic Cells And Natural Killer Cells In Response To Various Stimuli Such As Lps And Cytokines Involves A Nonconventional Secretory Process Via Secretory Lysosomes
Secreted By Plasma Cells In Response To Lps
Found On The Surface Of Activated Platelets
An Increased Chromatin Association Is Observed When Associated With The Adenovirus Protein Pvii
Protein Name High Mobility Group Protein B1
High Mobility Group Protein 1
Hmg-1
Alternative Names Anti-High Mobility Group Protein B1 antibody
Anti-High Mobility Group Protein 1 antibody
Anti-Hmg-1 antibody
Anti-HMGB1 antibody
Anti-HMG1 antibody
6 months standard