• Western blot analysis of lysates from COLO cells, using Histone H1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using Histone H1 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of HUVEC cells, using Histone H1 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of 3T3 cells using Histone H1 Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Western blot analysis of various cells using Histone H1 Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Immunofluorescence analysis of Hela cell. 1, Histone H1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Alpha-SMA monoclonal antibody (6A12) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Histone H1 antibody (1-50 aa) (STJ93515)

SKU:
STJ93515

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Histone H1.5 and Histone H1.3 and Histone H1.4 (1-50 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: H1-5
H1-3
3009
3007
3008
H15_HUMAN
H13_HUMAN
H14_HUMAN
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Immunogen Region: 1-50 aa
Specificity: Histone H1 Polyclonal Antibody detects endogenous levels of Histone H1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Histone H1 at the amino acid range 1-50
Post Translational Modifications H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter. Phosphorylated at Thr-11 by GSK3B during mitosis in prometaphase and dephosphorylated in telophase. Citrullination at Arg-57 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance.
Function Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.
Protein Name Histone H1.5
Histone H1a
Histone H1b
Histone H1s-3
Database Links Reactome: R-HSA-140342
Reactome: R-HSA-2559584
Cellular Localisation Nucleus
Chromosome
More Commonly Found In Heterochromatin
Associates With Actively Transcribed Chromatin And Not Heterochromatin
Alternative Antibody Names Anti-Histone H1.5 antibody
Anti-Histone H1a antibody
Anti-Histone H1b antibody
Anti-Histone H1s-3 antibody
Anti-H1-5 antibody
Anti-H1F5 antibody
Anti-HIST1H1B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance