• Western blot analysis of extracts of various cell lines, using HIRA antibody (STJ110759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of HIRA in paraffin-embedded human colon carcinoma using [KO Validated] HIRA Rabbit polyclonal antibody (STJ110759) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts from normal (control) and HIRA rabbit polyclonal antibody knockout (KO) 293T cells, using HIRA rabbit polyclonal antibody antibody (STJ110759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of HIRA in paraffin-embedded human lung cancer using [KO Validated] HIRA Rabbit polyclonal antibody (STJ110759) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human gastric cancer using HIRA antibody (STJ110759) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of HIRA in paraffin-embedded human gastric cancer using [KO Validated] HIRA Rabbit polyclonal antibody (STJ110759) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human lung cancer using HIRA antibody (STJ110759) at dilution of 1:100 (40x lens).
  • Western blot analysis of lysates from wild type (WT) and HIRA knockout (KO) 293T cells, using [KO Validated] HIRA Rabbit polyclonal antibody (STJ110759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using HIRA antibody (STJ110759) at dilution of 1:100 (40x lens).
  • Western blot analysis of various lysates using [KO Validated] HIRA Rabbit polyclonal antibody (STJ110759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HIRA antibody (250-550) (STJ110759)

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STJ110759

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HIRA (250-550) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HIRA
Gene ID: 7290
Uniprot ID: HIRA_HUMAN
Immunogen Region: 250-550
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 250-550 of human HIRA (NP_003316.3).
Immunogen Sequence: AQIIEREGWKTNMDFVGHRK AVTVVKFNPKIFKKKQKNGS SAKPSCPYCCCAVGSKDRSL SVWLTCLKRPLVVIHELFDK SIMDISWTLNGLGILVCSMD GSVAFLDFSQDELGDPLSEE EKSRIHQSTYGKSLAIMTEA QLSTAVIENPEMLKYQRRQQ QQQLDQKSAATREMGSATSV AGVVNGESLEDIRKNLLKKQ VETRTADGRRRITPLCIAQL DTGDFSTAFFNSIPLSGSL
Tissue Specificity Expressed at high levels in kidney, pancreas and skeletal muscle and at lower levels in brain, heart, liver, lung, and placenta.
Post Translational Modifications Sumoylated. Phosphorylated by CDK2/CCNA1 and CDK2/CCNE1 on Thr-555 in vitro. Also phosphorylated on Thr-555 and Ser-687 in vivo.
Function Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
Protein Name Protein Hira
Tup1-Like Enhancer Of Split Protein 1
Database Links Reactome: R-HSA-2559584
Cellular Localisation Nucleus
Pml Body
Primarily
Though Not Exclusively
Localized To The Nucleus
Localizes To Pml Bodies Immediately Prior To Onset Of Senescence
Alternative Antibody Names Anti-Protein Hira antibody
Anti-Tup1-Like Enhancer Of Split Protein 1 antibody
Anti-HIRA antibody
Anti-DGCR1 antibody
Anti-HIR antibody
Anti-TUPLE1 antibody

Information sourced from Uniprot.org

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