![Western blot analysis of various lysates using [KO Validated] GDI2 Rabbit pAb (STJ111343) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/91757/408642/STJ111343_1__69698.1744894972.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] GDI2 Rabbit pAb (STJ111343) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and GDI2 knockout (KO) 293T cells, using [KO Validated] GDI2 Rabbit pAb (STJ111343) at 1:2000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/91757/408643/STJ111343_2__70435.1744894972.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and GDI2 knockout (KO) 293T cells, using [KO Validated] GDI2 Rabbit pAb (STJ111343) at 1:2000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.")
![Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] GDI2 Rabbit pAb (STJ111343) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/91757/408644/STJ111343_3__65768.1744894973.jpg?c=1 "Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] GDI2 Rabbit pAb (STJ111343) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western blot analysis of various lysates using [KO Validated] GDI2 Rabbit pAb (STJ111343) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/91757/408642/STJ111343_1__69698.1744894972.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] GDI2 Rabbit pAb (STJ111343) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.")
Anti-GDI2 antibody (296-445) (STJ111343)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal GDI2 (296-445) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:200-1:3000IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GDI2 |
| Gene ID | 2665 |
| Uniprot ID | GDIB_HUMAN |
| Immunogen Region | 296-445 |
| Immunogen Sequence | QVIRVICILSHPIKNTNDAN SCQIIIPQNQVNRKSDIYVC MISFAHNVAAQGKYIAIVST TVETKEPEKEIRPALELLEP IEQKFVSISDLLVPKDLGTE SQIFISRTYDATTHFETTCD DIKNIYKRMTGSEFDFEEMK RKKNDIYGED |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 296-445 of human GDI2 (NP_001485.2). |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Function | GDP-dissociation inhibitor preventing the GDP to GTP exchange of most Rab proteins. By keeping these small GTPases in their inactive GDP-bound form regulates intracellular membrane trafficking. Negatively regulates protein transport to the cilium and ciliogenesis through the inhibition of RAB8A. |
| Protein Name | Rab Gdp Dissociation Inhibitor BetaRab Gdi BetaGuanosine Diphosphate Dissociation Inhibitor 2Gdi-2 |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-8876198 |
| Cellular Localisation | CytoplasmMembranePeripheral Membrane ProteinGolgi ApparatusTrans-Golgi Network |
| Alternative Antibody Names | Anti-Rab Gdp Dissociation Inhibitor Beta antibodyAnti-Rab Gdi Beta antibodyAnti-Guanosine Diphosphate Dissociation Inhibitor 2 antibodyAnti-Gdi-2 antibodyAnti-GDI2 antibodyAnti-RABGDIB antibody |
Information sourced from Uniprot.org