• STJ70842 (0.1µg/ml) staining of Human Liver (A), (0.03ug/ml) Testes (B) and Tonsil (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70842 (2µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
  • STJ70842 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and plasma membrane staining. The nucle
  • STJ70842 (0.1µg/ml) staining of Mouse Liver (A) and (0.03ug/ml) Rat Heart (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70842 (0.03µg/ml) staining of HeLa (A) and NIH3T3 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminesce
  • STJ70842 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and vesicle staining. The nuclear stai
  • STJ70842 (0.1µg/ml) staining of Human Liver (A), (0.03ug/ml) Testes (B) and Tonsil (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70842 (2µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
  • STJ70842 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and plasma membrane staining. The nucle
  • STJ70842 (0.1µg/ml) staining of Mouse Liver (A) and (0.03ug/ml) Rat Heart (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70842 (0.03µg/ml) staining of HeLa (A) and NIH3T3 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminesce
  • STJ70842 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and vesicle staining. The nuclear stai

Anti-GAPDH (Internal) Antibody (STJ70842)

SKU:
STJ70842
Current Stock:
Host: Goat
Applications: Pep-ELISA, WB, IHC, IF
Reactivity: Human, Mouse, Rat, Dog
Short Description: Goat polyclonal primary antibody against GAPDH (Internal).
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at-20°C. Minimize freezing and thawing.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage Instruction: Store at-20°C and avoid freeze-thaw cycles.
Concentration: 0.5mg/mL
Isotype: IgG
Dilution Range: WB-0.03-0.1µg/ml
IHC-2µg/ml
IF-Strong expression of the protein seen in the cytoplasm of U251 and HeLa cells. 5-10µg/ml
ELISA-antibody detection limit dilution 1:16000.
Specificity: This antibody is expected to recognize both reported isoforms (NP_002037.2; NP_001243728.1). Reported variants represent identical protein: NP_001276674.1, NP_002037.2, NP_001276675.1. GAPDH is constitutively expressed in almost all tissues at high l
Uniprot ID: G3P_MOUSE
Gene ID: 14433
Gene Symbol: Gapdh
Immunogen Region: internal region
Immunogen Sequence: GVNHEKYDNSLK
Accession Number: NP_002037.2; NP_001243728.1
6 months standard