• STJ70553 (1µg/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. <strong>This data is from a previous batch, not on sale.</strong>
  • STJ70553 (0.001µg/ml) staining of HEK293 (A) and HeLa (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70553 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and vesicle staining. The nuclear stai
  • STJ70553 (0.001µg/ml) staining of Human Liver (A), Tonsil (B) and (0.3ug/ml) Rat Brain (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence
  • STJ70553 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and vesicle staining. The nuclear stai
  • STJ70553 (1µg/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. <strong>This data is from a previous batch, not on sale.</strong>
  • STJ70553 (0.001µg/ml) staining of HEK293 (A) and HeLa (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70553 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and vesicle staining. The nuclear stai
  • STJ70553 (0.001µg/ml) staining of Human Liver (A), Tonsil (B) and (0.3ug/ml) Rat Brain (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence
  • STJ70553 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and vesicle staining. The nuclear stai

Anti-GAPDH (C Terminus) Loading Control Antibody (STJ70553)

SKU:
STJ70553
Current Stock:
Host: Goat
Applications: Pep-ELISA, WB, IF
Reactivity: Human, Mouse, Dog, Pig
Short Description: Goat polyclonal primary antibody against GAPDH (C Terminus) Loading Control.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at-20°C. Minimize freezing and thawing.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage Instruction: Store at-20°C and avoid freeze-thaw cycles.
Concentration: 0.5mg/mL
Isotype: IgG
Dilution Range: WB-0.001-0.003µg/ml
IF-Strong expression of the protein seen in the cytoplasm and vesicles of A549 and HeLa cells. 10µg/ml
ELISA-antibody detection limit dilution 1:128000.
Specificity: GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.
Uniprot ID: G3P_HUMAN
Gene ID: 2597
Gene Symbol: GAPDH
Immunogen Region: C Terminus 335
Immunogen Sequence: HQVVSSDFNSDT
Accession Number: NP_002037.2
6 months standard