• Western blot analysis of various cells with GAPDH antibody at dilution of 1:2000.
  • Immunohistochemistry of paraffin-embedded mouse kidney using GAPDH antibody at dilution of 1:500.
  • Immunohistochemistry of paraffin-embedded mouse testis using GAPDH antibody at dilution of 1:500.
  • Immunocytochemistry analysis of 4% paraformaldehyde fixed HeLa cells using GAPDH at dilution of 1:500.

Anti-GAPDH antibody [SR0338J] (STJA0005234)

Current Stock:
Host: Rabbit
Applications: WB, IHC, IF
Reactivity: Human, Mouse, Rat
Short Description: Rabbit monoclonal antibody anti-GAPDH is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: SR0338J
Conjugation: Unconjugated
Isotype: IgG1
Formulation: PBS with 0.02% Sodium Azide, 100 Mu g/mL BSA and 50% Glycerol.
Purification: Affinity purification
Concentration: 3 mg/ml
Storage Instruction: Store at-20C for one year. Avoid repeated freeze/thaw cycles.
Gene Symbol: Gapdh
Gene ID: 14433
Uniprot ID: G3P_MOUSE
Immunogen: KLH conjugated Synthetic peptide corresponding to Mouse GAPDH
Function Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC.
Protein Name Glyceraldehyde-3-Phosphate Dehydrogenase
Peptidyl-Cysteine S-Nitrosylase Gapdh
Database Links Reactome: R-MMU-70171
Reactome: -MMU-70263
Cellular Localisation Cytoplasm
Translocates To The Nucleus Following S-Nitrosylation And Interaction With Siah1
Which Contains A Nuclear Localization Signal
Colocalizes With Chp1 To Small Punctate Structures Along The Microtubules Tracks
Alternative Antibody Names Anti-Glyceraldehyde-3-Phosphate Dehydrogenase antibody
Anti-Gapdh antibody
Anti-Peptidyl-Cysteine S-Nitrosylase Gapdh antibody
Anti-Gapdh antibody
Anti-Gapd antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance