• Western blot analysis of the lysates from HUVECcells using FZD7 antibody.
  • Western blot analysis of lysates from HT-29 cells, using FZD7 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of HELA cells using Frizzled-7 Polyclonal Antibody diluted at 1:2000
  • Immunofluorescence analysis of MCF7 cells, using FZD7 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using Frizzled-7 Polyclonal Antibody diluted at 1:2000

Anti-Frizzled-7 antibody (STJ93151)

SKU:
STJ93151
Current Stock:
Host: Rabbit
Applications: WB, IF, ELISA
Reactivity: Human,Mouse
Short Description: Rabbit polyclonal primary antibody against Frizzled-7.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:20000
Specificity: Frizzled-7 polyclonal antibody detects endogenous levels of Frizzled-7 protein.
Uniprot ID: FZD7_HUMAN
Gene ID: 8324
Gene Symbol: FZD7
Immunogen: Synthesized peptide derived from human Frizzled-7
Immunogen Region: 20-100 aa, N-terminal
Tissue Specificity High expression in adult skeletal muscle and fetal kidney, followed by fetal lung, adult heart, brain, and placenta. Specifically expressed in squamous cell esophageal carcinomas.
Post Translational Modifications Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
Function Receptor for Wnt proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. Activation by WNT8 induces expression of beta-catenin target genes. Following ligand activation, binds to CCDC88C/DAPLE which displaces DVL1 from FZD7 and leads to inhibition of canonical Wnt signaling, activation of G-proteins by CCDC88C and triggering of non-canonical Wnt responses. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
Database Links Reactome: R-HSA-373080
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Endosome Membrane
Associated To The Plasma Membrane In The Presence Of Fzd7 And Phosphatidylinositol 4
5-Bisphosphate (Pip2)
Localized In Recycling Endosomes In Other Conditions
Protein Name Frizzled-7
Fz-7
Hfz7
Fze3
Alternative Names Anti-Frizzled-7 antibody
Anti-Fz-7 antibody
Anti-Hfz7 antibody
Anti-Fze3 antibody
Anti-FZD7 antibody
6 months standard