• Immunofluorescence analysis of COS7 cells, using FZD6 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-Frizzled-6 antibody (STJ93149)

SKU:
STJ93149
Current Stock:
Host: Rabbit
Applications: WB, IF, ELISA
Reactivity: Human
Short Description: Rabbit polyclonal primary antibody against Frizzled-6.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:5000
Specificity: Frizzled-6 polyclonal antibody detects endogenous levels of Frizzled-6 protein.
Uniprot ID: FZD6_HUMAN
Gene ID: 8323
Gene Symbol: FZD6
Immunogen: Synthesized peptide derived from human Frizzled-6
Immunogen Region: 90-170 aa, Internal
Tissue Specificity Detected in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, thymus, prostate, testis, ovary, small intestine and colon. In the fetus, expressed in brain, lung, liver and kidney.
Post Translational Modifications Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
Function Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Together with FZD3, is involved in the neural tube closure and plays a role in the regulation of the establishment of planar cell polarity (PCP), particularly in the orientation of asymmetric bundles of stereocilia on the apical faces of a subset of auditory and vestibular sensory cells located in the inner ear.
Database Links Reactome: R-HSA-373080
Cellular Localisation Membrane
Multi-Pass Membrane Protein
Cell Membrane
Cell Surface
Apical Cell Membrane
Cytoplasmic Vesicle Membrane
Endoplasmic Reticulum Membrane
Colocalizes With Fzd3 At The Apical Face Of Cells
Localizes To The Endoplasmic Reticulum Membrane In The Presence Of Lmbr1l
Protein Name Frizzled-6
Fz-6
Hfz6
Alternative Names Anti-Frizzled-6 antibody
Anti-Fz-6 antibody
Anti-Hfz6 antibody
Anti-FZD6 antibody

Information sourced from Uniprot.org

6 months standard