• Western blot analysis of lysates from LOVO cells, using FZD5 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of A549 cells, using FZD5 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-Frizzled-5 antibody (STJ93146)

SKU:
STJ93146
Current Stock:
Host: Rabbit
Applications: WB, IHC-P, IF, ELISA
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal primary antibody against Frizzled-5.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Specificity: Frizzled-5 polyclonal antibody detects endogenous levels of Frizzled-5 protein.
Uniprot ID: FZD5_HUMAN
Gene ID: 7855
Gene Symbol: FZD5
Immunogen: Synthesized peptide derived from human Frizzled-5
Immunogen Region: 440-520 aa, C-terminal
Post Translational Modifications Ubiquitinated by RNF43 and ZNRF3, leading to its degradation by the proteasome.
Function Receptor for Wnt proteins. Can activate WNT2, WNT10B, WNT5A, but not WNT2B or WNT4 (in vitro).the in vivo situation may be different since not all of these are known to be coexpressed. In neurons, activation of WNT7A promotes formation of synapses. Functions in the canonical Wnt/beta-catenin signaling pathway. The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable). Plays a role in yolk sac angiogenesis and in placental vascularization.
Database Links Reactome: R-HSA-373080
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Golgi Apparatus Membrane
Cell Junction
Synapse
Perikaryon
Cell Projection
Dendrite
Axon
Localized At The Plasma Membrane And Also Found At The Golgi
Protein Name Frizzled-5
Fz-5
Hfz5
Fze5
Alternative Names Anti-Frizzled-5 antibody
Anti-Fz-5 antibody
Anti-Hfz5 antibody
Anti-Fze5 antibody
Anti-FZD5 antibody
Anti-C2orf31 antibody

Information sourced from Uniprot.org

6 months standard