• Western blot analysis of lysates from HUVEC and COLO205 cells, using FGR Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using c-Fgr Polyclonal Antibody

Anti-FGR antibody (30-110 aa, Internal) (STJ92240)

SKU:
STJ92240

Current Stock:
Host: Rabbit
Applications: WB, IHC-P, ELISA
Reactivity: Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody Anti-Tyrosine-protein kinase Fgr (30-110 aa, Internal) is suitable for use in Western Blot, Immunohistochemistry and ELISA.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Concentration: 1 mg/ml
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Gene Symbol: FGR
Gene ID: 2268
Uniprot ID: FGR_HUMAN
Immunogen Region: 30-110 aa, Internal
Specificity: c-Fgr polyclonal antibody detects endogenous levels of c-Fgr protein.
Immunogen: Synthesized peptide derived from human c-Fgr
Post Translational Modifications Ubiquitinated. Becomes ubiquitinated in response to ITGB2 signaling.this does not lead to degradation. Phosphorylated. Autophosphorylated on tyrosine residues. Becomes phosphorylated in response to FCGR2A and/or FCGR2B engagement, cell adhesion and signaling by ITGB2. Prior phosphorylation at Tyr-523 by SRC inhibits ulterior autophosphorylation at Tyr-412.
Function Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors devoid of kinase activity and contributes to the regulation of immune responses, including neutrophil, monocyte, macrophage and mast cell functions, cytoskeleton remodeling in response to extracellular stimuli, phagocytosis, cell adhesion and migration. Promotes mast cell degranulation, release of inflammatory cytokines and IgE-mediated anaphylaxis. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as MS4A2/FCER1B, FCGR2A and/or FCGR2B. Acts downstream of ITGB1 and ITGB2, and regulates actin cytoskeleton reorganization, cell spreading and adhesion. Depending on the context, activates or inhibits cellular responses. Functions as negative regulator of ITGB2 signaling, phagocytosis and SYK activity in monocytes. Required for normal ITGB1 and ITGB2 signaling, normal cell spreading and adhesion in neutrophils and macrophages. Functions as positive regulator of cell migration and regulates cytoskeleton reorganization via RAC1 activation. Phosphorylates SYK (in vitro) and promotes SYK-dependent activation of AKT1 and MAP kinase signaling. Phosphorylates PLD2 in antigen-stimulated mast cells, leading to PLD2 activation and the production of the signaling molecules lysophosphatidic acid and diacylglycerol. Promotes activation of PIK3R1. Phosphorylates FASLG, and thereby regulates its ubiquitination and subsequent internalization. Phosphorylates ABL1. Promotes phosphorylation of CBL, CTTN, PIK3R1, PTK2/FAK1, PTK2B/PYK2 and VAV2. Phosphorylates HCLS1 that has already been phosphorylated by SYK, but not unphosphorylated HCLS1. Together with CLNK, it acts as a negative regulator of natural killer cell-activating receptors and inhibits interferon-gamma production.
Protein Name Tyrosine-Protein Kinase Fgr
Gardner-Rasheed Feline Sarcoma Viral
V-Fgr Oncogene Homolog
Proto-Oncogene C-Fgr
P55-Fgr
P58-Fgr
P58c-Fgr
Database Links Reactome: R-HSA-2029481
Reactome: R-HSA-432142
Reactome: R-HSA-6798695
Reactome: R-HSA-9664323
Reactome: R-HSA-9664422
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Peripheral Membrane Protein
Cell Projection
Ruffle Membrane
Cytoplasm
Cytosol
Cytoskeleton
Mitochondrion Inner Membrane
Mitochondrion Intermembrane Space
Detected In Mitochondrial Intermembrane Space And At Inner Membranes
Colocalizes With Actin Fibers At Membrane Ruffles
Detected At Plasma Membrane Lipid Rafts
Alternative Antibody Names Anti-Tyrosine-Protein Kinase Fgr antibody
Anti-Gardner-Rasheed Feline Sarcoma Viral antibody
Anti-V-Fgr Oncogene Homolog antibody
Anti-Proto-Oncogene C-Fgr antibody
Anti-P55-Fgr antibody
Anti-P58-Fgr antibody
Anti-P58c-Fgr antibody
Anti-FGR antibody
Anti-SRC2 antibody

Information sourced from Uniprot.org

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