• Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g EZH2/KMT6 antibody (STJ11100918). Western blot was performed from the immunoprecipitate using EZH2/KMT6 antibody (STJ11100918) at a dilution of 1:1000.
  • Immunohistochemistry analysis of EZH2/KMT6 in paraffin-embedded rat liver using EZH2/KMT6 Rabbit polyclonal antibody (STJ11100918) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of EZH2/KMT6 in paraffin-embedded mouse brain using EZH2/KMT6 Rabbit polyclonal antibody (STJ11100918) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of EZH2/KMT6 in paraffin-embedded human thyroid cancer using EZH2/KMT6 Rabbit polyclonal antibody (STJ11100918) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using EZH2/KMT6 Rabbit polyclonal antibody (STJ11100918) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-EZH2 antibody (133-252) (STJ11100918)

SKU:
STJ11100918

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EZH2 (133-252) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EZH2
Gene ID: 2146
Uniprot ID: EZH2_HUMAN
Immunogen Region: 133-252
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 133-252 of human EZH2/KMT6 (NP_001190176.1).
Immunogen Sequence: YMGDEVLDQDGTFIEELIKN YDGKVHGDRECGFINDEIFV ELVNALGQYNDDDDDDDGDD PEEREEKQKDLEDHRDDKES RPPRKFPSDKIFEAISSMFP DKGTAEELKEKYKELTEQQL
Tissue Specificity In the ovary, expressed in primordial follicles and oocytes and also in external follicle cells (at protein level). Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis.
Post Translational Modifications Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity. Phosphorylation at Thr-345 by CDK1 and CDK2 promotes maintenance of H3K27me3 levels at EZH2-target loci, thus leading to epigenetic gene silencing. Sumoylated. Glycosylated: O-GlcNAcylation at Ser-75 by OGT increases stability of EZH2 and facilitates the formation of H3K27me3 by the PRC2/EED-EZH2 complex.
Function Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2. Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-BMAL1 heterodimer.involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.
Protein Name Histone-Lysine N-Methyltransferase Ezh2
Enx-1
Enhancer Of Zeste Homolog 2
Lysine N-Methyltransferase 6
Database Links Reactome: R-HSA-212300
Reactome: R-HSA-2559580
Reactome: R-HSA-3214841
Reactome: R-HSA-5617472
Reactome: R-HSA-8943724
Reactome: R-HSA-8953750
Reactome: R-HSA-9609690
Reactome: R-HSA-9710421
Cellular Localisation Nucleus
Localizes To The Inactive X Chromosome In Trophoblast Stem Cells
Alternative Antibody Names Anti-Histone-Lysine N-Methyltransferase Ezh2 antibody
Anti-Enx-1 antibody
Anti-Enhancer Of Zeste Homolog 2 antibody
Anti-Lysine N-Methyltransferase 6 antibody
Anti-EZH2 antibody
Anti-KMT6 antibody

Information sourced from Uniprot.org

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