• Human breast infiltrating ductal carcinoma stained with anti-Her-2/neu antibody using peroxidase-conjugate and DAB chromogen. Note the strong membrane staining (3+) of carcinoma cells.

Anti-ERBB2 antibody [ZR5] (STJ180123)

SKU:
STJ180123

Current Stock:
Host: Rabbit
Applications: IHC-p
Reactivity: Human
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit monoclonal antibody Anti-ERBB2 is suitable for use in Immunohistochemistry.
Clonality: Monoclonal
Clone ID: ZR5
Conjugation: Unconjugated
Isotype: IgG
Formulation: Purified antibody diluted in Tris‐HCI buffer containing stabilizing protein and <0.1% sodium azide.
Dilution Range: 1:200‐300
Storage Instruction: Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C.
Gene Symbol: ERBB2
Gene ID: 2064
Uniprot ID: ERBB2_HUMAN
Immunogen: Recombinant protein encoding extracellular domain of human c‐erbB2
Tissue Specificity Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.
Post Translational Modifications Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues. Signaling via SEMA4C promotes phosphorylation at Tyr-1248. Dephosphorylated by PTPN12.
Function Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A.the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
Protein Name Receptor Tyrosine-Protein Kinase Erbb-2
Metastatic Lymph Node Gene 19 Protein
Mln 19
Proto-Oncogene Neu
Proto-Oncogene C-Erbb-2
Tyrosine Kinase-Type Cell Surface Receptor Her2
P185erbb2
Cd Antigen Cd340
Database Links Reactome: R-HSA-1227986
Reactome: R-HSA-1250196
Reactome: R-HSA-1251932
Reactome: R-HSA-1257604
Reactome: R-HSA-1306955
Reactome: R-HSA-1358803
Reactome: R-HSA-1963640
Reactome: R-HSA-1963642
Reactome: R-HSA-2219530
Reactome: R-HSA-416572
Reactome: R-HSA-5673001
Reactome: R-HSA-6785631
Reactome: R-HSA-6811558
Reactome: R-HSA-8847993
Reactome: R-HSA-8863795
Reactome: R-HSA-8866910
Reactome: R-HSA-9634285
Reactome: R-HSA-9652282
Reactome: R-HSA-9664565
Reactome: R-HSA-9665233
Reactome: R-HSA-9665244
Reactome: R-HSA-9665245
Reactome: R-HSA-9665246
Reactome: R-HSA-9665247
Reactome: R-HSA-9665249
Reactome: R-HSA-9665250
Reactome: R-HSA-9665251
Reactome: R-HSA-9665348
Reactome: R-HSA-9665686
Reactome: R-HSA-9665737
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Early Endosome
Cytoplasm
Perinuclear Region
Nucleus
Translocation To The Nucleus Requires Endocytosis
Probably Endosomal Sorting And Is Mediated By Importin Beta-1/Kpnb1
Also Detected In Vps35-Positive Endosome-To-Tgn Retrograde Vesicles
Isoform 2: Cytoplasm
Isoform 3: Cytoplasm
Alternative Antibody Names Anti-Receptor Tyrosine-Protein Kinase Erbb-2 antibody
Anti-Metastatic Lymph Node Gene 19 Protein antibody
Anti-Mln 19 antibody
Anti-Proto-Oncogene Neu antibody
Anti-Proto-Oncogene C-Erbb-2 antibody
Anti-Tyrosine Kinase-Type Cell Surface Receptor Her2 antibody
Anti-P185erbb2 antibody
Anti-Cd Antigen Cd340 antibody
Anti-ERBB2 antibody
Anti-HER2 antibody
Anti-MLN19 antibody
Anti-NEU antibody
Anti-NGL antibody

Information sourced from Uniprot.org

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